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- W2344342290 abstract "A total of 152 cows was examined in this study for Staphylococcus species, it was found that 44.3% of cows and were clinically mastitic whereas 14.5% were subclinically mastitic respectively. The identification of Staphylococcus species revealed that S. aureus, S. epidermidis, S. intermedius and S. hyicus for cows were (17.2%, 7.5%, 3.9% and 1.6%) respectively. Staphylococcus aureus isolates were confirmed after biochemical identification by API test. The study of virulence factors of total S. aureus isolates from mastitic cows revealed that lipase, fibrinolysin, DNase and protein A production were presented as percentage 67.3, 74.0, 85.6 and 84.6 respectively. The antibiotic sensitivity for S. aureus revealed that 96.2% of cow isolates were methicillin sensitive which considered the drug of choice for these isolates. The study also included the identification of S. aureus enterotoxins using set-RPLA and multiplex PCR. The incidence of enterotoxins C,A,B and D by set-RPLA were 36.5%, 14.4%, 10.6% and 2.9% respectively. Meanwhile the results of multiplex PCR were 7 isolates as enterotoxin C,4 isolates as enterotoxin E and one isolate for each A,B, and D respectively. The identification of MRSA of cow's isolates using PCR revealed that 3 isolates out of 5 isolates were positive. (El-Seedy,F.R, El-Shabrawy, M; Hakim, A. S; Dorgham,S.M.m Ata, S. Nagwa; Bakry, M.A and Osman,N.M.N. Recent Techniques used for Isolation and Characterization of Staphylococcus Aureus from Mastitic Cows. Journal of American Science 2010;6(12):701-708). (ISSN: 1545-1003). http://www.americanscience.org." @default.
- W2344342290 created "2016-06-24" @default.
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- W2344342290 date "2010-01-01" @default.
- W2344342290 modified "2023-09-26" @default.
- W2344342290 title "Recent Techniques used for Isolation and Characterization of Staphylococcus Aureus from Mastitic Cows." @default.
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