FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2344368445> ?p ?o ?g. }

• W2344368445 endingPage "4757" @default.
• W2344368445 startingPage "4744" @default.
• W2344368445 abstract "Damage-induced neuronal endopeptidase (DINE)/endothelin-converting enzyme-like 1 (ECEL1) is a membrane-bound metalloprotease, which we originally identified as a nerve regeneration-associated molecule. Abundant expression of DINE is observed in regenerating neurons, as well as in developing spinal motor neurons. In line with this, DINE -deficient ( DINE KO) embryos fail to arborize phrenic motor nerves in the diaphragm and to form proper neuromuscular junctions (NMJ), which lead to death shortly after birth. However, it is unclear whether protease activity of DINE is involved in motor nerve terminal arborization and how DINE participates in the process. To address these issues, we performed an in vivo rescue experiment in which three types of motor-neuron specific DINE transgenic mice were crossed with DINE KO mice. The DINE KO mice, which overexpressed wild-type DINE in motor neurons, succeeded in rescuing the aberrant nerve terminal arborization and lethality after birth, while those overexpressing two types of protease domain-mutated DINE failed. Further histochemical analysis showed abnormal behavior of immature Schwann cells along the DINE-deficient axons. Coculture experiments of motor neurons and Schwann cells ensured that the protease domain of neuronal DINE was required for proper alignment of immature Schwann cells along the axon. These findings suggest that protease activity of DINE is crucial for intramuscular innervation of motor nerves and subsequent NMJ formation, as well as proper control of interactions between axons and immature Schwann cells. SIGNIFICANCE STATEMENT Damage-induced neuronal endopeptidase (DINE) is a membrane-bound metalloprotease; expression is abundant in developing spinal motor neurons, as well as in nerve-injured neurons. DINE -deficient (KO) embryos fail to arborize phrenic motor nerves in the diaphragm and to form a neuromuscular junction, leading to death immediately after birth. To address whether proteolytic activity of DINE is involved in this process, we performed in vivo rescue experiments with DINE KO mice. Transgenic rescue of DINE KO mice was accomplished by overexpression of wild-type DINE, but not by protease domain-mutated DINE. Immature Schwann cells were abnormally aligned along the DINE protease-deficient axons. Thus, the protease activity of DINE is crucial for motor axon arborization, as well as the interaction between axons and immature Schwann cells." @default.
• W2344368445 created "2016-06-24" @default.
• W2344368445 creator A5009596447 @default.
• W2344368445 creator A5068238908 @default.
• W2344368445 creator A5075627852 @default.
• W2344368445 date "2016-04-27" @default.
• W2344368445 modified "2023-10-18" @default.
• W2344368445 title "Motor Nerve Arborization Requires Proteolytic Domain of Damage-Induced Neuronal Endopeptidase (DINE) during Development" @default.
• W2344368445 cites W1502037771 @default.
• W2344368445 cites W1510652713 @default.
• W2344368445 cites W1538232203 @default.
• W2344368445 cites W1549817168 @default.
• W2344368445 cites W1665419471 @default.
• W2344368445 cites W1860470769 @default.
• W2344368445 cites W1895241692 @default.
• W2344368445 cites W1919727069 @default.
• W2344368445 cites W1960355435 @default.
• W2344368445 cites W1963853740 @default.
• W2344368445 cites W1971001046 @default.
• W2344368445 cites W1977768257 @default.
• W2344368445 cites W1979983330 @default.
• W2344368445 cites W1980022376 @default.
• W2344368445 cites W1987552442 @default.
• W2344368445 cites W1995518203 @default.
• W2344368445 cites W1997946018 @default.
• W2344368445 cites W1998086237 @default.
• W2344368445 cites W1998208719 @default.
• W2344368445 cites W2000101032 @default.
• W2344368445 cites W2008588438 @default.
• W2344368445 cites W2014370614 @default.
• W2344368445 cites W2020919967 @default.
• W2344368445 cites W2021103771 @default.
• W2344368445 cites W2029212519 @default.
• W2344368445 cites W2031414088 @default.
• W2344368445 cites W2039431039 @default.
• W2344368445 cites W2041642022 @default.
• W2344368445 cites W2044199827 @default.
• W2344368445 cites W2063063761 @default.
• W2344368445 cites W2063791205 @default.
• W2344368445 cites W2064342341 @default.
• W2344368445 cites W2068488653 @default.
• W2344368445 cites W2070423416 @default.
• W2344368445 cites W2076828747 @default.
• W2344368445 cites W2079833508 @default.
• W2344368445 cites W2087248499 @default.
• W2344368445 cites W2092322285 @default.
• W2344368445 cites W2099882112 @default.
• W2344368445 cites W2118941096 @default.
• W2344368445 cites W2125307359 @default.
• W2344368445 cites W2128589265 @default.
• W2344368445 cites W2128686747 @default.
• W2344368445 cites W2131867298 @default.
• W2344368445 cites W2136903466 @default.
• W2344368445 cites W2139644337 @default.
• W2344368445 cites W2145807471 @default.
• W2344368445 cites W2153225946 @default.
• W2344368445 cites W2157042469 @default.
• W2344368445 cites W2158989452 @default.
• W2344368445 cites W2161961116 @default.
• W2344368445 cites W2162804111 @default.
• W2344368445 cites W2343097881 @default.
• W2344368445 doi "https://doi.org/10.1523/jneurosci.3811-15.2016" @default.
• W2344368445 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6601721" @default.
• W2344368445 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27122033" @default.
• W2344368445 hasPublicationYear "2016" @default.
• W2344368445 type Work @default.
• W2344368445 sameAs 2344368445 @default.
• W2344368445 citedByCount "13" @default.
• W2344368445 countsByYear W23443684452016 @default.
• W2344368445 countsByYear W23443684452017 @default.
• W2344368445 countsByYear W23443684452018 @default.
• W2344368445 countsByYear W23443684452019 @default.
• W2344368445 countsByYear W23443684452020 @default.
• W2344368445 countsByYear W23443684452022 @default.
• W2344368445 crossrefType "journal-article" @default.
• W2344368445 hasAuthorship W2344368445A5009596447 @default.
• W2344368445 hasAuthorship W2344368445A5068238908 @default.
• W2344368445 hasAuthorship W2344368445A5075627852 @default.
• W2344368445 hasBestOaLocation W23443684451 @default.
• W2344368445 hasConcept C109523444 @default.
• W2344368445 hasConcept C169760540 @default.
• W2344368445 hasConcept C181199279 @default.
• W2344368445 hasConcept C2775912687 @default.
• W2344368445 hasConcept C2776714187 @default.
• W2344368445 hasConcept C2776752467 @default.
• W2344368445 hasConcept C2777004073 @default.
• W2344368445 hasConcept C2777240379 @default.
• W2344368445 hasConcept C2779530196 @default.
• W2344368445 hasConcept C2780775167 @default.
• W2344368445 hasConcept C55493867 @default.
• W2344368445 hasConcept C55728118 @default.
• W2344368445 hasConcept C86803240 @default.
• W2344368445 hasConcept C95444343 @default.
• W2344368445 hasConceptScore W2344368445C109523444 @default.
• W2344368445 hasConceptScore W2344368445C169760540 @default.
• W2344368445 hasConceptScore W2344368445C181199279 @default.
• W2344368445 hasConceptScore W2344368445C2775912687 @default.
• W2344368445 hasConceptScore W2344368445C2776714187 @default.

• next