FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2344806730> ?p ?o ?g. }

• W2344806730 endingPage "384" @default.
• W2344806730 startingPage "373" @default.
• W2344806730 abstract "The role of hepatic triacylglycerol lipase (H-TGL) in promoting the liver uptake of high density lipoprotein (HDL) free and esterified cholesterol was studied in a recirculating rat liver perfusion, a situation where the enzyme is physiologically expressed and is active at the vascular bed. For this purpose, reconstituted HDL of defined phospholipid composition were prepared, containing either 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, a substrate for H-TGL, or 1-O-hexadecyl-2-oleoyl-sn-glycero-3-phosphocholine, a non-hydrolyzable analog. Reconstituted HDL were then used in the perfused rat liver system. The main results are the following. 1) Reconstituted HDL were obtained by sonication of lipids and apolipoproteins and isolated by ultracentrifugation in the 1.07-1.21 g/ml density interval. Reconstituted HDL containing either diacylphosphatidylcholine or alkyl-acyl-phosphatidylcholine were similar in terms of chemical composition, apparent size, and apolipoprotein A-I immunoreactivity, and were comparable to native HDL3. 2) Reconstituted HDL were labeled with free [14C]cholesterol and [3H]cholesteryl ether, a non-hydrolyzable tracer of esterified cholesterol, and were perfused through the rat liver. Liver uptake of [3H]cholesteryl ether was 2.5-fold higher from reconstituted HDL containing diacylphospholipid than from HDL reconstituted with alkyl-acyl-phospholipids. Liver uptake of free [14C]cholesterol was identical in both cases. 3) H-TGL-depleted rat livers were obtained by a 12-min preperfusion in the presence of heparin, displacing 90% of the enzymatic activity. The residual activity in the perfusate was inhibited by a specific antibody directed against rat H-TGL. Liver uptake of [3H]cholesteryl ether from reconstituted HDL containing diacylphospholipid was reduced by 35% in hepatic lipase-depleted livers compared to controls. On the other hand, hepatic lipase depletion had no effect on the liver uptake of esterified cholesterol from HDL reconstituted with alkyl-acyl-phospholipids. The above findings support a role for the phospholipase A1 activity of H-TGL in stimulating the delivery of HDL esterified cholesterol to liver cells." @default.
• W2344806730 created "2016-06-24" @default.
• W2344806730 creator A5002010497 @default.
• W2344806730 creator A5012269350 @default.
• W2344806730 creator A5016622325 @default.
• W2344806730 creator A5055141217 @default.
• W2344806730 creator A5061349365 @default.
• W2344806730 creator A5064849353 @default.
• W2344806730 date "1994-03-01" @default.
• W2344806730 modified "2023-10-15" @default.
• W2344806730 title "Hepatic lipase promotes the uptake of HDL esterified cholesterol by the perfused rat liver: a study using reconstituted HDL particles of defined phospholipid composition." @default.
• W2344806730 cites W1487993287 @default.
• W2344806730 cites W1503830583 @default.
• W2344806730 cites W1515091337 @default.
• W2344806730 cites W1523684025 @default.
• W2344806730 cites W1531185348 @default.
• W2344806730 cites W1544118301 @default.
• W2344806730 cites W1548991782 @default.
• W2344806730 cites W1560257150 @default.
• W2344806730 cites W1582749228 @default.
• W2344806730 cites W1601120722 @default.
• W2344806730 cites W1775749144 @default.
• W2344806730 cites W1835984024 @default.
• W2344806730 cites W1910326869 @default.
• W2344806730 cites W1971456096 @default.
• W2344806730 cites W1975816765 @default.
• W2344806730 cites W1983370217 @default.
• W2344806730 cites W1986654080 @default.
• W2344806730 cites W1988010122 @default.
• W2344806730 cites W1989713074 @default.
• W2344806730 cites W1994163364 @default.
• W2344806730 cites W1997396883 @default.
• W2344806730 cites W2006261853 @default.
• W2344806730 cites W2016445477 @default.
• W2344806730 cites W2018746110 @default.
• W2344806730 cites W2021703905 @default.
• W2344806730 cites W2023492391 @default.
• W2344806730 cites W2032938715 @default.
• W2344806730 cites W2040206216 @default.
• W2344806730 cites W2046346838 @default.
• W2344806730 cites W2062867348 @default.
• W2344806730 cites W2083446323 @default.
• W2344806730 cites W2084963543 @default.
• W2344806730 cites W2085597192 @default.
• W2344806730 cites W2144220657 @default.
• W2344806730 cites W2185261374 @default.
• W2344806730 cites W2300552860 @default.
• W2344806730 cites W2324176011 @default.
• W2344806730 cites W2339873058 @default.
• W2344806730 cites W2412834615 @default.
• W2344806730 cites W41165184 @default.
• W2344806730 doi "https://doi.org/10.1016/s0022-2275(20)41190-3" @default.
• W2344806730 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8014574" @default.
• W2344806730 hasPublicationYear "1994" @default.
• W2344806730 type Work @default.
• W2344806730 sameAs 2344806730 @default.
• W2344806730 citedByCount "58" @default.
• W2344806730 countsByYear W23448067302018 @default.
• W2344806730 crossrefType "journal-article" @default.
• W2344806730 hasAuthorship W2344806730A5002010497 @default.
• W2344806730 hasAuthorship W2344806730A5012269350 @default.
• W2344806730 hasAuthorship W2344806730A5016622325 @default.
• W2344806730 hasAuthorship W2344806730A5055141217 @default.
• W2344806730 hasAuthorship W2344806730A5061349365 @default.
• W2344806730 hasAuthorship W2344806730A5064849353 @default.
• W2344806730 hasBestOaLocation W23448067301 @default.
• W2344806730 hasConcept C110459506 @default.
• W2344806730 hasConcept C126322002 @default.
• W2344806730 hasConcept C134018914 @default.
• W2344806730 hasConcept C138885662 @default.
• W2344806730 hasConcept C181199279 @default.
• W2344806730 hasConcept C185592680 @default.
• W2344806730 hasConcept C2778163477 @default.
• W2344806730 hasConcept C2778918659 @default.
• W2344806730 hasConcept C2779697368 @default.
• W2344806730 hasConcept C2780072125 @default.
• W2344806730 hasConcept C2780213950 @default.
• W2344806730 hasConcept C40231798 @default.
• W2344806730 hasConcept C41625074 @default.
• W2344806730 hasConcept C41895202 @default.
• W2344806730 hasConcept C55493867 @default.
• W2344806730 hasConcept C71924100 @default.
• W2344806730 hasConcept C86803240 @default.
• W2344806730 hasConceptScore W2344806730C110459506 @default.
• W2344806730 hasConceptScore W2344806730C126322002 @default.
• W2344806730 hasConceptScore W2344806730C134018914 @default.
• W2344806730 hasConceptScore W2344806730C138885662 @default.
• W2344806730 hasConceptScore W2344806730C181199279 @default.
• W2344806730 hasConceptScore W2344806730C185592680 @default.
• W2344806730 hasConceptScore W2344806730C2778163477 @default.
• W2344806730 hasConceptScore W2344806730C2778918659 @default.
• W2344806730 hasConceptScore W2344806730C2779697368 @default.
• W2344806730 hasConceptScore W2344806730C2780072125 @default.
• W2344806730 hasConceptScore W2344806730C2780213950 @default.
• W2344806730 hasConceptScore W2344806730C40231798 @default.
• W2344806730 hasConceptScore W2344806730C41625074 @default.
• W2344806730 hasConceptScore W2344806730C41895202 @default.
• W2344806730 hasConceptScore W2344806730C55493867 @default.

• next