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- W2344952200 abstract "Previous studies have shown that Nicotiana tabacum contains three Agrobacterium-derived T-DNA sequences inherited from its paternal ancestor Nicotiana tomentosiformis. Among these, the TB locus carries an intact mannopine synthase 2′ gene (TB-mas2′). This gene is similar to the Agrobacterium rhizogenes A4-mas2′ gene that encodes the synthesis of the Amadori compound deoxyfructosyl-glutamine (DFG or santhopine). In this study we show that TB-mas2′ is expressed at very low levels in N. tomentosiformis and in most N. tabacum cultivars; however, some cultivars show high TB-mas2′ expression levels. The TB-mas2′ promoter sequences of low- and high-expressing cultivars are identical. The low/high level of expression segregates as a single Mendelian factor in a cross between a low- and a high-expression cultivar. pTB-mas2′-GUS and pA4-mas2′-GUS reporter genes were stably introduced in N. benthamiana. Both were mainly expressed in the root expansion zone and leaf vasculature. Roots of tobacco cultivars with high TB-mas2′ expression contain detectable levels of DFG." @default.
- W2344952200 created "2016-06-24" @default.
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- W2344952200 date "2016-07-26" @default.
- W2344952200 modified "2023-10-02" @default.
- W2344952200 title "Root-specific expression of opine genes and opine accumulation in some cultivars of the naturally occurring genetically modified organism<i>Nicotiana tabacum</i>" @default.
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- W2344952200 doi "https://doi.org/10.1111/tpj.13196" @default.
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