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- W2345313413 abstract "To investigate the function of 14-3-3 protein in response to excess nitrate stress, a 14-3-3 protein, designated as So14-3-3, was isolated from spinach. Phylogenetic analysis demonstrated that So14-3-3 belongs to non-ε group of 14-3-3 superfamily. Real time-quantitative RT-PCR and western blot analysis showed that So14-3-3 was induced by excess nitrate stress in spinach roots and leaves. After nitrate treatment, the phosphorylated H+-ATPase and nitrate reductase (NR) increased and decreased respectively. Co-Immunoprecipitation (Co-IP) suggested that the interaction of So14-3-3 with the phosphorylated H+-ATPase enhanced, but reduced with phosphorylated NR in spinach roots after nitrate treatment. Besides, 5 proteins interacted with So14-3-3 were found by Co-IP and LC-MS/MS analysis. So14-3-3 overexpressing transgenic tobacco plants showed enhanced tolerance to nitrate treatment at the germination and young seedlings stage. The transgenic plants showed longer root length, lower malondialdehyde (MDA), H2O2, protein carbonyl contents, relatively higher soluble sugar and protein contents, than the WT plants after nitrate treatment. The phosphorylation levels of H+-ATPase in transgenic plants were higher than the WT plants after nitrate treatment, whereas NR were lower. Additionally, in transgenic plants, the interaction of So14-3-3 with phosphorylated H+-ATPase and NR increased and decreased more than the WT plants under nitrate stress, leading to higher H+-ATPase and NR activities in transgenic plants. These data suggested that So14-3-3 might be involved in nitrate stress response by interacting with H+-ATPase and NR." @default.
- W2345313413 created "2016-06-24" @default.
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- W2345313413 date "2016-09-01" @default.
- W2345313413 modified "2023-10-11" @default.
- W2345313413 title "Spinach 14-3-3 protein interacts with the plasma membrane H+-ATPase and nitrate reductase in response to excess nitrate stress" @default.
- W2345313413 cites W1569287373 @default.
- W2345313413 cites W1966048054 @default.
- W2345313413 cites W1977472221 @default.
- W2345313413 cites W1980526825 @default.
- W2345313413 cites W1983165669 @default.
- W2345313413 cites W1989060957 @default.
- W2345313413 cites W1989088302 @default.
- W2345313413 cites W1989410111 @default.
- W2345313413 cites W1993149181 @default.
- W2345313413 cites W1994361064 @default.
- W2345313413 cites W1995473255 @default.
- W2345313413 cites W1995961381 @default.
- W2345313413 cites W1997760983 @default.
- W2345313413 cites W1997940063 @default.
- W2345313413 cites W1999623913 @default.
- W2345313413 cites W2002370642 @default.
- W2345313413 cites W2003282114 @default.
- W2345313413 cites W2007787620 @default.
- W2345313413 cites W2010318953 @default.
- W2345313413 cites W2011631607 @default.
- W2345313413 cites W2011954737 @default.
- W2345313413 cites W2016958628 @default.
- W2345313413 cites W2022028228 @default.
- W2345313413 cites W2024670108 @default.
- W2345313413 cites W2031492820 @default.
- W2345313413 cites W2033430187 @default.
- W2345313413 cites W2040010685 @default.
- W2345313413 cites W2042122216 @default.
- W2345313413 cites W2045263649 @default.
- W2345313413 cites W2046363884 @default.
- W2345313413 cites W2062620524 @default.
- W2345313413 cites W2069319059 @default.
- W2345313413 cites W2070271353 @default.
- W2345313413 cites W2071924923 @default.
- W2345313413 cites W2077895194 @default.
- W2345313413 cites W2079402776 @default.
- W2345313413 cites W2081213526 @default.
- W2345313413 cites W2082091267 @default.
- W2345313413 cites W2082987551 @default.
- W2345313413 cites W2085136652 @default.
- W2345313413 cites W2088885333 @default.
- W2345313413 cites W2094139666 @default.
- W2345313413 cites W2096381222 @default.
- W2345313413 cites W2097191318 @default.
- W2345313413 cites W2098535539 @default.
- W2345313413 cites W2102227865 @default.
- W2345313413 cites W2107857664 @default.
- W2345313413 cites W2108066735 @default.
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- W2345313413 cites W2131965028 @default.
- W2345313413 cites W2157451936 @default.
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- W2345313413 cites W4293247451 @default.
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- W2345313413 doi "https://doi.org/10.1016/j.plaphy.2016.04.043" @default.
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