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- W2345476987 endingPage "507" @default.
- W2345476987 startingPage "491" @default.
- W2345476987 abstract "Single molecule methods have yielded new insight into how RecA filaments form and find homologous DNA. RecA nucleation and growth on SSB-coated ssDNA is kinetically regulated by the RecFOR and RecOR complexes, which both microscopically and macroscopically alter the SSB–ssDNA nucleoprotein fiber. RecA finds homology through many random, weak, and transient interactions made in parallel and ignores very short regions of microhomology resulting in a reduction of both dimensionality and complexity. Homologous recombination maintains genomic integrity by repairing broken chromosomes. The broken chromosome is partially resected to produce single-stranded DNA (ssDNA) that is used to search for homologous double-stranded DNA (dsDNA). This homology driven ‘search and rescue’ is catalyzed by a class of DNA strand exchange proteins that are defined in relation to Escherichia coli RecA, which forms a filament on ssDNA. Here, we review the regulation of RecA filament assembly and the mechanism by which RecA quickly and efficiently searches for and identifies a unique homologous sequence among a vast excess of heterologous DNA. Given that RecA is the prototypic DNA strand exchange protein, its behavior affords insight into the actions of eukaryotic RAD51 orthologs and their regulators, BRCA2 and other tumor suppressors. Homologous recombination maintains genomic integrity by repairing broken chromosomes. The broken chromosome is partially resected to produce single-stranded DNA (ssDNA) that is used to search for homologous double-stranded DNA (dsDNA). This homology driven ‘search and rescue’ is catalyzed by a class of DNA strand exchange proteins that are defined in relation to Escherichia coli RecA, which forms a filament on ssDNA. Here, we review the regulation of RecA filament assembly and the mechanism by which RecA quickly and efficiently searches for and identifies a unique homologous sequence among a vast excess of heterologous DNA. Given that RecA is the prototypic DNA strand exchange protein, its behavior affords insight into the actions of eukaryotic RAD51 orthologs and their regulators, BRCA2 and other tumor suppressors. RecA: Regulation and Mechanism of a Molecular Search Engine: (Trends in Biochemical Sciences, June 2016, Vol. 41, No. 6, 491--507)Bell et al.Trends in Biochemical SciencesJune 6, 2016In BriefDue to an oversight in the preparation of this Review article, the title and legend for Supplemental Video S1 were inadvertently omitted. The Supplemental Video S1 title and legend is given below, and can now be found in the online version of the article. The authors and TiBS regret any confusion this may have caused. Full-Text PDF" @default.
- W2345476987 created "2016-06-24" @default.
- W2345476987 creator A5002209510 @default.
- W2345476987 creator A5032365839 @default.
- W2345476987 date "2016-06-01" @default.
- W2345476987 modified "2023-10-18" @default.
- W2345476987 title "RecA: Regulation and Mechanism of a Molecular Search Engine" @default.
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