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• W2345582536 abstract "Microorganisms are a rich source of bioactives; however, chemical identification is a major bottleneck. Strategies that can prioritize the most prolific microbial strains and novel compounds are of great interest. Here, we present an integrated approach to evaluate the biosynthetic richness in bacteria and mine the associated chemical diversity. Thirteen strains closely related to Pseudoalteromonas luteoviolacea isolated from all over the Earth were analyzed using an untargeted metabolomics strategy, and metabolomic profiles were correlated with whole-genome sequences of the strains. We found considerable diversity: only 2% of the chemical features and 7% of the biosynthetic genes were common to all strains, while 30% of all features and 24% of the genes were unique to single strains. The list of chemical features was reduced to 50 discriminating features using a genetic algorithm and support vector machines. Features were dereplicated by tandem mass spectrometry (MS/MS) networking to identify molecular families of the same biosynthetic origin, and the associated pathways were probed using comparative genomics. Most of the discriminating features were related to antibacterial compounds, including the thiomarinols that were reported from P. luteoviolacea here for the first time. By comparative genomics, we identified the biosynthetic cluster responsible for the production of the antibiotic indolmycin, which could not be predicted with standard methods. In conclusion, we present an efficient, integrative strategy for elucidating the chemical richness of a given set of bacteria and link the chemistry to biosynthetic genes. IMPORTANCE We here combine chemical analysis and genomics to probe for new bioactive secondary metabolites based on their pattern of distribution within bacterial species. We demonstrate the usefulness of this combined approach in a group of marine Gram-negative bacteria closely related to Pseudoalteromonas luteoviolacea, which is a species known to produce a broad spectrum of chemicals. The approach allowed us to identify new antibiotics and their associated biosynthetic pathways. Combining chemical analysis and genetics is an efficient mining workflow for identifying diverse pharmaceutical candidates in a broad range of microorganisms and therefore of great use in bioprospecting." @default.
• W2345582536 created "2016-06-24" @default.
• W2345582536 creator A5015192553 @default.
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• W2345582536 creator A5054589286 @default.
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• W2345582536 creator A5086663128 @default.
• W2345582536 date "2016-06-28" @default.
• W2345582536 modified "2023-09-26" @default.
• W2345582536 title "An Integrated Metabolomic and Genomic Mining Workflow To Uncover the Biosynthetic Potential of Bacteria" @default.
• W2345582536 cites W143096088 @default.
• W2345582536 cites W1577208830 @default.
• W2345582536 cites W1812763490 @default.
• W2345582536 cites W1963649489 @default.
• W2345582536 cites W1966156878 @default.
• W2345582536 cites W1968166882 @default.
• W2345582536 cites W1968216644 @default.
• W2345582536 cites W1968248059 @default.
• W2345582536 cites W1968252650 @default.
• W2345582536 cites W1970413517 @default.
• W2345582536 cites W1972675532 @default.
• W2345582536 cites W1972960408 @default.
• W2345582536 cites W1975478536 @default.
• W2345582536 cites W1975775976 @default.
• W2345582536 cites W1976113023 @default.
• W2345582536 cites W1976172994 @default.
• W2345582536 cites W1976578940 @default.
• W2345582536 cites W1978503036 @default.
• W2345582536 cites W1979663509 @default.
• W2345582536 cites W1984355417 @default.
• W2345582536 cites W1989299822 @default.
• W2345582536 cites W1997562548 @default.
• W2345582536 cites W1998834021 @default.
• W2345582536 cites W1999846232 @default.
• W2345582536 cites W2001872883 @default.
• W2345582536 cites W2003906118 @default.
• W2345582536 cites W2008353386 @default.
• W2345582536 cites W2012960603 @default.
• W2345582536 cites W2019176012 @default.
• W2345582536 cites W2022960083 @default.
• W2345582536 cites W2025108364 @default.
• W2345582536 cites W2025437701 @default.
• W2345582536 cites W2025901385 @default.
• W2345582536 cites W2026974870 @default.
• W2345582536 cites W2030972600 @default.
• W2345582536 cites W2041537722 @default.
• W2345582536 cites W2043364538 @default.
• W2345582536 cites W2043472907 @default.
• W2345582536 cites W2049735624 @default.
• W2345582536 cites W2053300710 @default.
• W2345582536 cites W2055615325 @default.
• W2345582536 cites W2063653245 @default.
• W2345582536 cites W2064452752 @default.
• W2345582536 cites W2065532765 @default.
• W2345582536 cites W2069969741 @default.
• W2345582536 cites W2073482539 @default.
• W2345582536 cites W2074633350 @default.
• W2345582536 cites W2075109094 @default.
• W2345582536 cites W2078395913 @default.
• W2345582536 cites W2080046774 @default.
• W2345582536 cites W2081418090 @default.
• W2345582536 cites W2081465448 @default.
• W2345582536 cites W2082315335 @default.
• W2345582536 cites W2084375915 @default.
• W2345582536 cites W2085450812 @default.
• W2345582536 cites W2092097556 @default.
• W2345582536 cites W2100195325 @default.
• W2345582536 cites W2101650875 @default.
• W2345582536 cites W2106017862 @default.
• W2345582536 cites W2114754207 @default.
• W2345582536 cites W2114853012 @default.
• W2345582536 cites W2117054393 @default.
• W2345582536 cites W2118766743 @default.
• W2345582536 cites W2119999417 @default.
• W2345582536 cites W2124148699 @default.
• W2345582536 cites W2125088742 @default.
• W2345582536 cites W2131711237 @default.
• W2345582536 cites W2135639274 @default.
• W2345582536 cites W2137659876 @default.
• W2345582536 cites W2142678478 @default.
• W2345582536 cites W2144803530 @default.
• W2345582536 cites W2145837008 @default.
• W2345582536 cites W2145974700 @default.
• W2345582536 cites W2149777566 @default.
• W2345582536 cites W2150550043 @default.
• W2345582536 cites W2152907639 @default.
• W2345582536 cites W2155142785 @default.
• W2345582536 cites W2157923841 @default.
• W2345582536 cites W2167054778 @default.
• W2345582536 cites W2171745213 @default.
• W2345582536 cites W2171908015 @default.
• W2345582536 cites W2618025358 @default.
• W2345582536 cites W2951007634 @default.
• W2345582536 doi "https://doi.org/10.1128/msystems.00028-15" @default.

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