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- W2345793136 abstract "A concise review o f iron ligands p resen tly u sed for the determ ination of serum iron and its ancillary unsaturated iron b in d in g capacity is p resen ted . T he review includes a discussion o f critical areas involved in iron de term i nations affecting the use of these ligands such as serum blanking , stand ardization, and the effect of b lanking on standardization; m ethod com plex ity; m olar absorptiv ities; po ten tial in terferences; choice o f reducing agent and procedural sensitivity according to selected d ilu tion conditions along w ith sam ple and physiological variables as exem plified by d iu rnal varia tions, and posture effects; volum e exclusion effects as a function of serum treatm ent; high lip id concentration as a volum e effect; therapeu tic in te r ferences, hem olysis, irre levant absorption, and jaund ice ; and th e use of m olar absorptivity as a quality control standardization check. From several possible choices, one sensitive ligand, 2,4-bis(5,6-diphenyll,2,4-triazin-3-yl) pyrid ine tetrasulfonate, is se lec ted w hich is su itab le for contem porary filtrate m ethodology and needs. A procedure is described d e ta iling its use for the determ ination o f serum iron in the routine m ode. The determ ination o f iron-b ind ing capacity is also included for the sake of com p leteness of the procedure. I f desired , the ligand selected here can be substitu ted for less sensitive ligands in o ther d irec t determ inations, m anual or robotized, or in continuous flow spectrophotom etry for e ith er d irec t or dialysate technology because the com pound reacts in a sim ilar m anner to the ligands that are presently acceptable in the fie ld of serum iron and ironb ind ing capacity quantification. * Supported in part by a Grant-in-Aid from Detroit Receiving Hospital Research Corporation." @default.
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- W2345793136 date "1980-07-01" @default.
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- W2345793136 title "Modern iron ligands useful for the measurement of serum iron." @default.
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