FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2345996245> ?p ?o ?g. }

• W2345996245 endingPage "280" @default.
• W2345996245 startingPage "277" @default.
• W2345996245 abstract "In order to survive, bacteria continually sense, and respond to, environmental fluctuations. Stringent control represents a key bacterial stress response to nutrient starvation that leads to rapid and comprehensive reprogramming of metabolic and transcriptional patterns. In general, transcription of genes for growth and proliferation is downregulated, while those important for survival and virulence are upregulated. Amino acid starvation is sensed by depletion of the aminoacylated tRNA pools, and this results in accumulation of ribosomes stalled with non-aminoacylated (uncharged) tRNA in the ribosomal A site. RelA is recruited to stalled ribosomes and activated to synthesize a hyperphosphorylated guanosine analogue, (p)ppGpp, which acts as a pleiotropic secondary messenger. However, structural information about how RelA recognizes stalled ribosomes and discriminates against aminoacylated tRNAs is missing. Here we present the cryo-electron microscopy structure of RelA bound to the bacterial ribosome stalled with uncharged tRNA. The structure reveals that RelA utilizes a distinct binding site compared to the translational factors, with a multi-domain architecture that wraps around a highly distorted A-site tRNA. The TGS (ThrRS, GTPase and SpoT) domain of RelA binds the CCA tail to orient the free 3' hydroxyl group of the terminal adenosine towards a β-strand, such that an aminoacylated tRNA at this position would be sterically precluded. The structure supports a model in which association of RelA with the ribosome suppresses auto-inhibition to activate synthesis of (p)ppGpp and initiate the stringent response. Since stringent control is responsible for the survival of pathogenic bacteria under stress conditions, and contributes to chronic infections and antibiotic tolerance, RelA represents a good target for the development of novel antibacterial therapeutics." @default.
• W2345996245 created "2016-06-24" @default.
• W2345996245 creator A5027897860 @default.
• W2345996245 creator A5073767436 @default.
• W2345996245 creator A5080012276 @default.
• W2345996245 creator A5086176987 @default.
• W2345996245 date "2016-05-09" @default.
• W2345996245 modified "2023-10-17" @default.
• W2345996245 title "Ribosome-dependent activation of stringent control" @default.
• W2345996245 cites W1488674567 @default.
• W2345996245 cites W1528632786 @default.
• W2345996245 cites W1530131939 @default.
• W2345996245 cites W1580190049 @default.
• W2345996245 cites W1597428765 @default.
• W2345996245 cites W1964979713 @default.
• W2345996245 cites W1971507610 @default.
• W2345996245 cites W2004252769 @default.
• W2345996245 cites W2006257982 @default.
• W2345996245 cites W2017016334 @default.
• W2345996245 cites W2022754910 @default.
• W2345996245 cites W2027025154 @default.
• W2345996245 cites W2029582401 @default.
• W2345996245 cites W2031404312 @default.
• W2345996245 cites W2031880123 @default.
• W2345996245 cites W2034876354 @default.
• W2345996245 cites W2047745452 @default.
• W2345996245 cites W2050952012 @default.
• W2345996245 cites W2051957156 @default.
• W2345996245 cites W2052915258 @default.
• W2345996245 cites W2054511300 @default.
• W2345996245 cites W2055114795 @default.
• W2345996245 cites W2058875960 @default.
• W2345996245 cites W2064578221 @default.
• W2345996245 cites W2073824449 @default.
• W2345996245 cites W2080528351 @default.
• W2345996245 cites W2086812659 @default.
• W2345996245 cites W2097604487 @default.
• W2345996245 cites W2098943921 @default.
• W2345996245 cites W2100455255 @default.
• W2345996245 cites W2103903051 @default.
• W2345996245 cites W2104234755 @default.
• W2345996245 cites W2120396539 @default.
• W2345996245 cites W2124026197 @default.
• W2345996245 cites W2129775801 @default.
• W2345996245 cites W2132629607 @default.
• W2345996245 cites W2136017260 @default.
• W2345996245 cites W2142672816 @default.
• W2345996245 cites W2146801942 @default.
• W2345996245 cites W2149360962 @default.
• W2345996245 cites W2150790075 @default.
• W2345996245 cites W2152562710 @default.
• W2345996245 cites W2154714625 @default.
• W2345996245 cites W2155201844 @default.
• W2345996245 cites W2164856850 @default.
• W2345996245 cites W2165048399 @default.
• W2345996245 cites W2168332319 @default.
• W2345996245 cites W2398117144 @default.
• W2345996245 cites W2950967305 @default.
• W2345996245 doi "https://doi.org/10.1038/nature17675" @default.
• W2345996245 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4900451" @default.
• W2345996245 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27279228" @default.
• W2345996245 hasPublicationYear "2016" @default.
• W2345996245 type Work @default.
• W2345996245 sameAs 2345996245 @default.
• W2345996245 citedByCount "178" @default.
• W2345996245 countsByYear W23459962452016 @default.
• W2345996245 countsByYear W23459962452017 @default.
• W2345996245 countsByYear W23459962452018 @default.
• W2345996245 countsByYear W23459962452019 @default.
• W2345996245 countsByYear W23459962452020 @default.
• W2345996245 countsByYear W23459962452021 @default.
• W2345996245 countsByYear W23459962452022 @default.
• W2345996245 countsByYear W23459962452023 @default.
• W2345996245 crossrefType "journal-article" @default.
• W2345996245 hasAuthorship W2345996245A5027897860 @default.
• W2345996245 hasAuthorship W2345996245A5073767436 @default.
• W2345996245 hasAuthorship W2345996245A5080012276 @default.
• W2345996245 hasAuthorship W2345996245A5086176987 @default.
• W2345996245 hasBestOaLocation W23459962452 @default.
• W2345996245 hasConcept C104317684 @default.
• W2345996245 hasConcept C105580179 @default.
• W2345996245 hasConcept C106774688 @default.
• W2345996245 hasConcept C149364088 @default.
• W2345996245 hasConcept C153957851 @default.
• W2345996245 hasConcept C185592680 @default.
• W2345996245 hasConcept C26628138 @default.
• W2345996245 hasConcept C3675279 @default.
• W2345996245 hasConcept C547475151 @default.
• W2345996245 hasConcept C55493867 @default.
• W2345996245 hasConcept C67705224 @default.
• W2345996245 hasConcept C86803240 @default.
• W2345996245 hasConcept C88478588 @default.
• W2345996245 hasConcept C95444343 @default.
• W2345996245 hasConceptScore W2345996245C104317684 @default.
• W2345996245 hasConceptScore W2345996245C105580179 @default.
• W2345996245 hasConceptScore W2345996245C106774688 @default.
• W2345996245 hasConceptScore W2345996245C149364088 @default.
• W2345996245 hasConceptScore W2345996245C153957851 @default.

• next