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- W2346206331 abstract "Introduction: For the rat pancreas it has been shown that endothelin (ET-1, ET-2, ET-3) mediates vasoconstriction with consequent deterioration of the microcirculation and recruitment of leukocytes. Selective inhibition of the ETA receptor reduces the pancreatitis-associated microcirculatory failure and emigration of leukocytes into pancreatic tissue. It is not known, however, which of the three endothelins plays a key role in the recruitment of pancreatic leukocytes. Therefore, we have investigate endothelin-stimulated activation of pancreatic endothelial cells and its association to leukocyte adhesion in pancreatic postcapillary venules. Experiments were carried out by using intravital microscopy. Methods: For intravital microscopy 50 male Sprague Dawley rats were anesthetized with sodium pentobarbital (50 mg/kg i. p.) followed by a laparotomy and exterioration of the pancreas. Administration of ET-1, ET-2 or ET-3 (1, 10, 100 pmol, n = 5/group) was carried out by local superfusion, while the control animals received 0.9 % NaCl solution (n = 5) only. Using rodamin 6G-stained leukocytes and FITC-stained latex beads (diameter 1 µm), post capillary leukocyte-adhesion and endothelial cell activation was investigated by intravital microscopy. Mean ± SEM, ANOVA and Student–Newman–Keuls test. Results: In comparison with baseline conditions and the control group all three endothelins cause a significant (p < 0.05) an dose-dependent increase in postcapillary leukocyte adhesion. In each group (ET-1, ET-2, ET-3) the most pronounced effect was seen with 100 pmol (ET-1: 859 ± 230 cells/mm 2 ; ET-2: 688 ± 112 cells/mm 2 , ET-3: 815 ± 114 cells/mm2, control: 258 ± 48 cells/mm2). In contrast, assessment of endothelial cell activation using FITC-stained latex beads did not show any dose- dependency and the maximum effect was already found with the smallest dosage of 1 pmol ((ET-1 (1 pmol): 7.9 ± 0.4 beads/mm 2 , ET-2 (1 pmol): 8.4 ± 0.3 beads/mm 2 , ET-3 (1 pmol): 8.9 ± 0.1 beads/mm 2 versus control: 5.9 ± 0.1 beads/mm 2 ; p < 0.05). Furthermore, no correlation between activation of endothelial-cells and leukocyte adhesion could be determined. Conclusion: The endothelin-stimulated activation of leukocyte-endothelial cell interaction is strictly dosedependent. However, we could not detect a suspected difference between the three endothelins. Furthermore, the endothelin-stimulated activation of endothelial cells, which was observed by adhesion unspecific latex-beads, did not correlate with the dose-dependent adhesion of leukocytes. This indicates that the adhesion of leukocytes is not solely triggered by the activation of endothelial cells." @default.
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- W2346206331 date "2006-01-01" @default.
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- W2346206331 title "Die Endothelin-vermittelte Endothelzellaktivierung bestimmt nicht ausschließlich die Leukozyten-Endothelzell-Interaktion in der pankreatischen Mikrostrombahn Endothelin-induced endothelial cell activation is not the only factor triggering not only leukocyte - endothelial cell interaction in the pancreatic microvascular system" @default.
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