FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2346396160> ?p ?o ?g. }

• W2346396160 endingPage "e2183" @default.
• W2346396160 startingPage "e2183" @default.
• W2346396160 abstract "The baculovirus expression vector system (BEVS) has been widely used to produce a large number of recombinant proteins, and is becoming one of the most powerful, robust, and cost-effective systems for the production of eukaryotic proteins. Nevertheless, as in any other protein expression system, it is important to improve the production capabilities of this vector. The orf46 viral gene was identified among the most highly abundant sequences in the transcriptome of Spodoptera exigua larvae infected with its native baculovirus, the S. exigua multiple nucleopolyhedrovirus (SeMNPV). Different sequences upstream of the orf46 gene were cloned, and their promoter activities were tested by the expression of the GFP reporter gene using the Autographa californica nucleopolyhedrovirus (AcMNPV) vector system in different insect cell lines (Sf21, Se301, and Hi5) and in larvae from S. exigua and Trichoplusia ni . The strongest promoter activity was defined by a 120 nt sequence upstream of the ATG start codon for the orf46 gene. On average, GFP expression under this new promoter was more than two fold higher than the expression obtained with the standard polyhedrin (polh) promoter. Additionally, the orf46 promoter was also tested in combination with the polh promoter, revealing an additive effect over the polh promoter activity. In conclusion, this new characterized promoter represents an excellent alternative to the most commonly used baculovirus promoters for the efficient expression of recombinant proteins using the BEVS." @default.
• W2346396160 created "2016-06-24" @default.
• W2346396160 creator A5002127331 @default.
• W2346396160 creator A5007364208 @default.
• W2346396160 creator A5044936034 @default.
• W2346396160 creator A5056000940 @default.
• W2346396160 creator A5087724635 @default.
• W2346396160 date "2016-06-28" @default.
• W2346396160 modified "2023-10-18" @default.
• W2346396160 title "A novel baculovirus-derived promoter with high activity in the baculovirus expression system" @default.
• W2346396160 cites W1519911846 @default.
• W2346396160 cites W1577043467 @default.
• W2346396160 cites W1581899920 @default.
• W2346396160 cites W1588679159 @default.
• W2346396160 cites W184607450 @default.
• W2346396160 cites W1969331007 @default.
• W2346396160 cites W1976468619 @default.
• W2346396160 cites W1976957104 @default.
• W2346396160 cites W1984985875 @default.
• W2346396160 cites W2002141015 @default.
• W2346396160 cites W2008758235 @default.
• W2346396160 cites W2013135488 @default.
• W2346396160 cites W2015820134 @default.
• W2346396160 cites W2020250129 @default.
• W2346396160 cites W2027339061 @default.
• W2346396160 cites W2046034094 @default.
• W2346396160 cites W2051208609 @default.
• W2346396160 cites W2053263670 @default.
• W2346396160 cites W2054351352 @default.
• W2346396160 cites W2059791019 @default.
• W2346396160 cites W2059907723 @default.
• W2346396160 cites W2064129235 @default.
• W2346396160 cites W2078292305 @default.
• W2346396160 cites W2078424920 @default.
• W2346396160 cites W2081685459 @default.
• W2346396160 cites W2083177459 @default.
• W2346396160 cites W2091479268 @default.
• W2346396160 cites W2091841873 @default.
• W2346396160 cites W2106476476 @default.
• W2346396160 cites W2135670032 @default.
• W2346396160 cites W2159424407 @default.
• W2346396160 cites W2170293208 @default.
• W2346396160 cites W2171369144 @default.
• W2346396160 cites W3022454936 @default.
• W2346396160 cites W89127838 @default.
• W2346396160 doi "https://doi.org/10.7717/peerj.2183" @default.
• W2346396160 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4928464" @default.
• W2346396160 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27375973" @default.
• W2346396160 hasPublicationYear "2016" @default.
• W2346396160 type Work @default.
• W2346396160 sameAs 2346396160 @default.
• W2346396160 citedByCount "15" @default.
• W2346396160 countsByYear W23463961602016 @default.
• W2346396160 countsByYear W23463961602017 @default.
• W2346396160 countsByYear W23463961602018 @default.
• W2346396160 countsByYear W23463961602019 @default.
• W2346396160 countsByYear W23463961602021 @default.
• W2346396160 countsByYear W23463961602022 @default.
• W2346396160 countsByYear W23463961602023 @default.
• W2346396160 crossrefType "journal-article" @default.
• W2346396160 hasAuthorship W2346396160A5002127331 @default.
• W2346396160 hasAuthorship W2346396160A5007364208 @default.
• W2346396160 hasAuthorship W2346396160A5044936034 @default.
• W2346396160 hasAuthorship W2346396160A5056000940 @default.
• W2346396160 hasAuthorship W2346396160A5087724635 @default.
• W2346396160 hasBestOaLocation W23463961601 @default.
• W2346396160 hasConcept C101762097 @default.
• W2346396160 hasConcept C103878061 @default.
• W2346396160 hasConcept C104317684 @default.
• W2346396160 hasConcept C105580179 @default.
• W2346396160 hasConcept C142613039 @default.
• W2346396160 hasConcept C150194340 @default.
• W2346396160 hasConcept C153911025 @default.
• W2346396160 hasConcept C161733203 @default.
• W2346396160 hasConcept C173758957 @default.
• W2346396160 hasConcept C176990463 @default.
• W2346396160 hasConcept C2777212814 @default.
• W2346396160 hasConcept C2777773057 @default.
• W2346396160 hasConcept C2778350275 @default.
• W2346396160 hasConcept C2778457844 @default.
• W2346396160 hasConcept C2779341487 @default.
• W2346396160 hasConcept C2780199244 @default.
• W2346396160 hasConcept C2781140520 @default.
• W2346396160 hasConcept C33161422 @default.
• W2346396160 hasConcept C40767141 @default.
• W2346396160 hasConcept C54355233 @default.
• W2346396160 hasConcept C59822182 @default.
• W2346396160 hasConcept C86803240 @default.
• W2346396160 hasConceptScore W2346396160C101762097 @default.
• W2346396160 hasConceptScore W2346396160C103878061 @default.
• W2346396160 hasConceptScore W2346396160C104317684 @default.
• W2346396160 hasConceptScore W2346396160C105580179 @default.
• W2346396160 hasConceptScore W2346396160C142613039 @default.
• W2346396160 hasConceptScore W2346396160C150194340 @default.
• W2346396160 hasConceptScore W2346396160C153911025 @default.
• W2346396160 hasConceptScore W2346396160C161733203 @default.
• W2346396160 hasConceptScore W2346396160C173758957 @default.
• W2346396160 hasConceptScore W2346396160C176990463 @default.

• next