FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2346668818> ?p ?o ?g. }

• W2346668818 endingPage "299.e4" @default.
• W2346668818 startingPage "290" @default.
• W2346668818 abstract "BackgroundIncreasing evidence suggests that the low-affinity receptor for IgE, CD23, plays an important role in controlling the activity of allergen-specific T cells through IgE-facilitated allergen presentation.ObjectiveWe sought to determine the number of CD23 molecules on immune cells in allergic patients and to investigate whether the number of CD23 molecules on antigen-presenting cells is associated with IgE levels and influences allergen uptake and allergen-specific T-cell activation.MethodsNumbers of CD23 molecules on immune cells of allergic patients were quantified by using flow cytometry with QuantiBRITE beads and compared with total and allergen-specific IgE levels, as well as with allergen-induced immediate skin reactivity. Allergen uptake and allergen-specific T-cell activation in relation to CD23 surface density were determined by using flow cytometry in combination with confocal microscopy and T cells transfected with the T-cell receptor specific for the birch pollen allergen Bet v 1, respectively. Defined IgE-allergen immune complexes were formed with human monoclonal allergen-specific IgE and Bet v 1.ResultsIn allergic patients the vast majority of CD23 molecules were expressed on naive IgD+ B cells. The density of CD23 molecules on B cells but not the number of CD23+ cells correlated with total IgE levels (RS = 0.53, P = .03) and allergen-induced skin reactions (RS = 0.63, P = .008). Uptake of allergen-IgE complexes into B cells and activation of allergen-specific T cells depended on IgE binding to CD23 and were associated with CD23 surface density. Addition of monoclonal IgE to cultured PBMCs significantly (P = .04) increased CD23 expression on B cells.ConclusionCD23 surface density on B cells of allergic patients is correlated with allergen-specific IgE levels and determines allergen uptake and subsequent activation of T cells. Increasing evidence suggests that the low-affinity receptor for IgE, CD23, plays an important role in controlling the activity of allergen-specific T cells through IgE-facilitated allergen presentation. We sought to determine the number of CD23 molecules on immune cells in allergic patients and to investigate whether the number of CD23 molecules on antigen-presenting cells is associated with IgE levels and influences allergen uptake and allergen-specific T-cell activation. Numbers of CD23 molecules on immune cells of allergic patients were quantified by using flow cytometry with QuantiBRITE beads and compared with total and allergen-specific IgE levels, as well as with allergen-induced immediate skin reactivity. Allergen uptake and allergen-specific T-cell activation in relation to CD23 surface density were determined by using flow cytometry in combination with confocal microscopy and T cells transfected with the T-cell receptor specific for the birch pollen allergen Bet v 1, respectively. Defined IgE-allergen immune complexes were formed with human monoclonal allergen-specific IgE and Bet v 1. In allergic patients the vast majority of CD23 molecules were expressed on naive IgD+ B cells. The density of CD23 molecules on B cells but not the number of CD23+ cells correlated with total IgE levels (RS = 0.53, P = .03) and allergen-induced skin reactions (RS = 0.63, P = .008). Uptake of allergen-IgE complexes into B cells and activation of allergen-specific T cells depended on IgE binding to CD23 and were associated with CD23 surface density. Addition of monoclonal IgE to cultured PBMCs significantly (P = .04) increased CD23 expression on B cells. CD23 surface density on B cells of allergic patients is correlated with allergen-specific IgE levels and determines allergen uptake and subsequent activation of T cells." @default.
• W2346668818 created "2016-06-24" @default.
• W2346668818 creator A5009312574 @default.
• W2346668818 creator A5016261280 @default.
• W2346668818 creator A5018719393 @default.
• W2346668818 creator A5019980273 @default.
• W2346668818 creator A5044428473 @default.
• W2346668818 creator A5050048947 @default.
• W2346668818 creator A5074453312 @default.
• W2346668818 creator A5076166428 @default.
• W2346668818 creator A5081407448 @default.
• W2346668818 creator A5081929224 @default.
• W2346668818 date "2017-01-01" @default.
• W2346668818 modified "2023-10-17" @default.
• W2346668818 title "CD23 surface density on B cells is associated with IgE levels and determines IgE-facilitated allergen uptake, as well as activation of allergen-specific T cells" @default.
• W2346668818 cites W111255074 @default.
• W2346668818 cites W1592386122 @default.
• W2346668818 cites W1774240040 @default.
• W2346668818 cites W1906224138 @default.
• W2346668818 cites W1911765136 @default.
• W2346668818 cites W1913366718 @default.
• W2346668818 cites W1939108455 @default.
• W2346668818 cites W1970594645 @default.
• W2346668818 cites W1973688027 @default.
• W2346668818 cites W1975780852 @default.
• W2346668818 cites W2000252764 @default.
• W2346668818 cites W2003055823 @default.
• W2346668818 cites W2005851413 @default.
• W2346668818 cites W2006880284 @default.
• W2346668818 cites W2009818128 @default.
• W2346668818 cites W2018522670 @default.
• W2346668818 cites W2026119244 @default.
• W2346668818 cites W2029130004 @default.
• W2346668818 cites W2035845602 @default.
• W2346668818 cites W2043559809 @default.
• W2346668818 cites W2047494851 @default.
• W2346668818 cites W2052063445 @default.
• W2346668818 cites W2059084455 @default.
• W2346668818 cites W2059618810 @default.
• W2346668818 cites W2064180188 @default.
• W2346668818 cites W2066555469 @default.
• W2346668818 cites W2074191073 @default.
• W2346668818 cites W2075056701 @default.
• W2346668818 cites W2078146109 @default.
• W2346668818 cites W2083672696 @default.
• W2346668818 cites W2088197448 @default.
• W2346668818 cites W2102751976 @default.
• W2346668818 cites W2104772500 @default.
• W2346668818 cites W2105126460 @default.
• W2346668818 cites W2116368130 @default.
• W2346668818 cites W2116583914 @default.
• W2346668818 cites W2123733113 @default.
• W2346668818 cites W2129457993 @default.
• W2346668818 cites W2133005443 @default.
• W2346668818 cites W2137558208 @default.
• W2346668818 cites W2146045461 @default.
• W2346668818 cites W2147246695 @default.
• W2346668818 cites W2166467474 @default.
• W2346668818 cites W2186540453 @default.
• W2346668818 doi "https://doi.org/10.1016/j.jaci.2016.03.042" @default.
• W2346668818 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5321593" @default.
• W2346668818 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27372566" @default.
• W2346668818 hasPublicationYear "2017" @default.
• W2346668818 type Work @default.
• W2346668818 sameAs 2346668818 @default.
• W2346668818 citedByCount "56" @default.
• W2346668818 countsByYear W23466688182017 @default.
• W2346668818 countsByYear W23466688182018 @default.
• W2346668818 countsByYear W23466688182019 @default.
• W2346668818 countsByYear W23466688182020 @default.
• W2346668818 countsByYear W23466688182021 @default.
• W2346668818 countsByYear W23466688182022 @default.
• W2346668818 countsByYear W23466688182023 @default.
• W2346668818 crossrefType "journal-article" @default.
• W2346668818 hasAuthorship W2346668818A5009312574 @default.
• W2346668818 hasAuthorship W2346668818A5016261280 @default.
• W2346668818 hasAuthorship W2346668818A5018719393 @default.
• W2346668818 hasAuthorship W2346668818A5019980273 @default.
• W2346668818 hasAuthorship W2346668818A5044428473 @default.
• W2346668818 hasAuthorship W2346668818A5050048947 @default.
• W2346668818 hasAuthorship W2346668818A5074453312 @default.
• W2346668818 hasAuthorship W2346668818A5076166428 @default.
• W2346668818 hasAuthorship W2346668818A5081407448 @default.
• W2346668818 hasAuthorship W2346668818A5081929224 @default.
• W2346668818 hasBestOaLocation W23466688181 @default.
• W2346668818 hasConcept C141105273 @default.
• W2346668818 hasConcept C159654299 @default.
• W2346668818 hasConcept C185592680 @default.
• W2346668818 hasConcept C203014093 @default.
• W2346668818 hasConcept C207480886 @default.
• W2346668818 hasConcept C2780510475 @default.
• W2346668818 hasConcept C34814297 @default.
• W2346668818 hasConcept C553184892 @default.
• W2346668818 hasConcept C86803240 @default.
• W2346668818 hasConcept C8891405 @default.
• W2346668818 hasConceptScore W2346668818C141105273 @default.
• W2346668818 hasConceptScore W2346668818C159654299 @default.
• W2346668818 hasConceptScore W2346668818C185592680 @default.

• next