FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2346827768> ?p ?o ?g. }

• W2346827768 abstract "Given its high surplus and low cost, glycerol has emerged as interesting carbon substrate for the synthesis of value-added chemicals. The soil bacterium Pseudomonas putida KT2440 can use glycerol to synthesize medium-chain-length poly(3-hydroxyalkanoates) (mcl-PHA), a class of biopolymers of industrial interest. Here, glycerol metabolism in P. putida KT2440 was studied on the level of gene expression (transcriptome) and metabolic fluxes (fluxome), using precisely adjusted chemostat cultures, growth kinetics and stoichiometry, to gain a systematic understanding of the underlying metabolic and regulatory network. Glycerol-grown P. putida KT2440 has a maintenance energy requirement [0.039 (mmolglycerol (gCDW h)−1)] that is about sixteen times lower than that of other bacteria, such as Escherichia coli, which provides a great advantage to use this substrate commercially. The shift from carbon (glycerol) to nitrogen (ammonium) limitation drives the modulation of specific genes involved in glycerol metabolism, transport electron chain, sensors to assess the energy level of the cell, and PHA synthesis, as well as changes in flux distribution to increase the precursor availability for PHA synthesis (Entner–Doudoroff pathway and pyruvate metabolism) and to reduce respiration (glyoxylate shunt). Under PHA-producing conditions (N-limitation), a higher PHA yield was achieved at low dilution rate (29.7 wt% of CDW) as compared to a high rate (12.8 wt% of CDW). By-product formation (succinate, malate) was specifically modulated under these regimes. On top of experimental data, elementary flux mode analysis revealed the metabolic potential of P. putida KT2440 to synthesize PHA and identified metabolic engineering targets towards improved production performance on glycerol. This study revealed the complex interplay of gene expression levels and metabolic fluxes under PHA- and non-PHA producing conditions using the attractive raw material glycerol as carbon substrate. This knowledge will form the basis for the development of future metabolically engineered hyper-PHA-producing strains derived from the versatile bacterium P. putida KT2440." @default.
• W2346827768 created "2016-06-24" @default.
• W2346827768 creator A5071093246 @default.
• W2346827768 creator A5075432861 @default.
• W2346827768 creator A5078145813 @default.
• W2346827768 creator A5084674318 @default.
• W2346827768 date "2016-05-03" @default.
• W2346827768 modified "2023-10-14" @default.
• W2346827768 title "Integrated analysis of gene expression and metabolic fluxes in PHA-producing Pseudomonas putida grown on glycerol" @default.
• W2346827768 cites W1497101056 @default.
• W2346827768 cites W1527577753 @default.
• W2346827768 cites W1546952499 @default.
• W2346827768 cites W1549469339 @default.
• W2346827768 cites W1549936434 @default.
• W2346827768 cites W1604790719 @default.
• W2346827768 cites W1933372907 @default.
• W2346827768 cites W1937279395 @default.
• W2346827768 cites W1964227455 @default.
• W2346827768 cites W1964245273 @default.
• W2346827768 cites W1972990890 @default.
• W2346827768 cites W1977125035 @default.
• W2346827768 cites W1979287129 @default.
• W2346827768 cites W1989488173 @default.
• W2346827768 cites W1990524871 @default.
• W2346827768 cites W1990931150 @default.
• W2346827768 cites W1991888393 @default.
• W2346827768 cites W1993756952 @default.
• W2346827768 cites W1996912752 @default.
• W2346827768 cites W2002077151 @default.
• W2346827768 cites W2004097455 @default.
• W2346827768 cites W2008788121 @default.
• W2346827768 cites W2015038163 @default.
• W2346827768 cites W2015751324 @default.
• W2346827768 cites W2020279574 @default.
• W2346827768 cites W2029705967 @default.
• W2346827768 cites W2033227888 @default.
• W2346827768 cites W2035457828 @default.
• W2346827768 cites W2036449301 @default.
• W2346827768 cites W2039371539 @default.
• W2346827768 cites W2044143765 @default.
• W2346827768 cites W2049494658 @default.
• W2346827768 cites W2052145892 @default.
• W2346827768 cites W2054920102 @default.
• W2346827768 cites W2060337613 @default.
• W2346827768 cites W2060649687 @default.
• W2346827768 cites W2063792424 @default.
• W2346827768 cites W2064455390 @default.
• W2346827768 cites W2067218839 @default.
• W2346827768 cites W2072543338 @default.
• W2346827768 cites W2073914246 @default.
• W2346827768 cites W2076048194 @default.
• W2346827768 cites W2077820538 @default.
• W2346827768 cites W2080329944 @default.
• W2346827768 cites W2095763963 @default.
• W2346827768 cites W2095773862 @default.
• W2346827768 cites W2095863530 @default.
• W2346827768 cites W2099039198 @default.
• W2346827768 cites W2099720039 @default.
• W2346827768 cites W2103576591 @default.
• W2346827768 cites W2108403115 @default.
• W2346827768 cites W2108786034 @default.
• W2346827768 cites W2110355460 @default.
• W2346827768 cites W2117282430 @default.
• W2346827768 cites W2122023734 @default.
• W2346827768 cites W2122598723 @default.
• W2346827768 cites W2123060905 @default.
• W2346827768 cites W2123687537 @default.
• W2346827768 cites W2124724728 @default.
• W2346827768 cites W2126593014 @default.
• W2346827768 cites W2126849248 @default.
• W2346827768 cites W2127384727 @default.
• W2346827768 cites W2130397266 @default.
• W2346827768 cites W2133505559 @default.
• W2346827768 cites W2139542233 @default.
• W2346827768 cites W2144503445 @default.
• W2346827768 cites W2148779442 @default.
• W2346827768 cites W2152211203 @default.
• W2346827768 cites W2152280797 @default.
• W2346827768 cites W2155250997 @default.
• W2346827768 cites W2156527767 @default.
• W2346827768 cites W2159010530 @default.
• W2346827768 cites W2159096710 @default.
• W2346827768 cites W2164216268 @default.
• W2346827768 cites W2165684405 @default.
• W2346827768 cites W2169903193 @default.
• W2346827768 cites W2171219265 @default.
• W2346827768 cites W2172287360 @default.
• W2346827768 cites W2324174866 @default.
• W2346827768 cites W2461074713 @default.
• W2346827768 cites W4242557011 @default.
• W2346827768 doi "https://doi.org/10.1186/s12934-016-0470-2" @default.
• W2346827768 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4855977" @default.
• W2346827768 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27142075" @default.
• W2346827768 hasPublicationYear "2016" @default.
• W2346827768 type Work @default.
• W2346827768 sameAs 2346827768 @default.
• W2346827768 citedByCount "67" @default.
• W2346827768 countsByYear W23468277682016 @default.
• W2346827768 countsByYear W23468277682017 @default.
• W2346827768 countsByYear W23468277682018 @default.

• next