Matches in SemOpenAlex for { <https://semopenalex.org/work/W2347048785> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W2347048785 endingPage "S104" @default.
- W2347048785 startingPage "S104" @default.
- W2347048785 abstract "Introduction The beneficial effects of hyperbaric oxygen (HBO) in the amelioration of ischemia-reperfusion (IR) injury have been shown to occur through a nitric oxide-dependent mechanism. Previous work has shown an early increase in eNOS activity caused by HBO. There is also a late up-regulation of eNOS mRNA. The purpose of this experiment is to determine if there is an increase in eNOS protein following HBO treatment. α2-Antiplasmin (α2-antiplasmin) was administered to determine the source of eNOS. Methods The gracilis muscle flap was raised in 40 male Wistar rats (n = 10/group). Rats were divided into four groups: a nonischemic control, IR, IR + HBO (100% O2 at 2.5 ATA) and an IR + HBO + α2-antiplasmin group each with an n = 10. Ischemia consisted of 4 hours of global ischemia on the gracilis muscle flap. The α2-antiplasmin was administered by direct continuous infusion into the gracilis muscle prior to HBO. The HBO treatment consisted 100% O2 at 2.5 ATA during the last 90 minutes of ischemia. The gracilis muscle and pulmonary vasculature were harvested 30 minutes after reperfusion, weighed, and flash frozen in liquid nitrogen. A Bradford assay was performed to determine the total protein quantity. The quantity of eNOS protein was measured using Western immunoblotting. Results IR did not significantly increase eNOS protein compared with the nonischemic control (35.6 ± 1.3 vs 38.6 ± 1.7 ng). HBO did not change the level of eNOS protein in skeletal muscle (38.8 ± 2.0 ng). In addition, the blockade of the plasmin/eNOS cascade had no effect on the level of eNOS protein (35.8 ± 2.7 ng). Discussions These results offer evidence that the immediate increase in eNOS activity seen in HBO treated IR injury is not due to an increase in eNOS protein. Instead, it may be related to an increase in VEGF protein or mechanotransduction of the VEGFR2 caused by the sheer stress of reperfusion." @default.
- W2347048785 created "2016-06-24" @default.
- W2347048785 creator A5004736858 @default.
- W2347048785 creator A5018915182 @default.
- W2347048785 creator A5038103148 @default.
- W2347048785 creator A5043239386 @default.
- W2347048785 creator A5044839866 @default.
- W2347048785 creator A5086191665 @default.
- W2347048785 date "2007-01-01" @default.
- W2347048785 modified "2023-09-25" @default.
- W2347048785 title "THE EFFECT OF HYPERBARIC OXYGEN ON ENDOTHELIAL NITRIC OXIDE SYNTHASE EXPRESSION IN ISCHEMIA-REPERFUSION INJURY." @default.
- W2347048785 doi "https://doi.org/10.1097/00042871-200701010-00177" @default.
- W2347048785 hasPublicationYear "2007" @default.
- W2347048785 type Work @default.
- W2347048785 sameAs 2347048785 @default.
- W2347048785 citedByCount "0" @default.
- W2347048785 crossrefType "journal-article" @default.
- W2347048785 hasAuthorship W2347048785A5004736858 @default.
- W2347048785 hasAuthorship W2347048785A5018915182 @default.
- W2347048785 hasAuthorship W2347048785A5038103148 @default.
- W2347048785 hasAuthorship W2347048785A5043239386 @default.
- W2347048785 hasAuthorship W2347048785A5044839866 @default.
- W2347048785 hasAuthorship W2347048785A5086191665 @default.
- W2347048785 hasConcept C126322002 @default.
- W2347048785 hasConcept C178790620 @default.
- W2347048785 hasConcept C185592680 @default.
- W2347048785 hasConcept C2777622882 @default.
- W2347048785 hasConcept C2778326061 @default.
- W2347048785 hasConcept C2780114680 @default.
- W2347048785 hasConcept C2992676626 @default.
- W2347048785 hasConcept C2994405533 @default.
- W2347048785 hasConcept C42219234 @default.
- W2347048785 hasConcept C519581460 @default.
- W2347048785 hasConcept C540031477 @default.
- W2347048785 hasConcept C541997718 @default.
- W2347048785 hasConcept C71924100 @default.
- W2347048785 hasConcept C98274493 @default.
- W2347048785 hasConceptScore W2347048785C126322002 @default.
- W2347048785 hasConceptScore W2347048785C178790620 @default.
- W2347048785 hasConceptScore W2347048785C185592680 @default.
- W2347048785 hasConceptScore W2347048785C2777622882 @default.
- W2347048785 hasConceptScore W2347048785C2778326061 @default.
- W2347048785 hasConceptScore W2347048785C2780114680 @default.
- W2347048785 hasConceptScore W2347048785C2992676626 @default.
- W2347048785 hasConceptScore W2347048785C2994405533 @default.
- W2347048785 hasConceptScore W2347048785C42219234 @default.
- W2347048785 hasConceptScore W2347048785C519581460 @default.
- W2347048785 hasConceptScore W2347048785C540031477 @default.
- W2347048785 hasConceptScore W2347048785C541997718 @default.
- W2347048785 hasConceptScore W2347048785C71924100 @default.
- W2347048785 hasConceptScore W2347048785C98274493 @default.
- W2347048785 hasIssue "1" @default.
- W2347048785 hasLocation W23470487851 @default.
- W2347048785 hasOpenAccess W2347048785 @default.
- W2347048785 hasPrimaryLocation W23470487851 @default.
- W2347048785 hasRelatedWork W2018797644 @default.
- W2347048785 hasRelatedWork W2079776789 @default.
- W2347048785 hasRelatedWork W2090126574 @default.
- W2347048785 hasRelatedWork W2319824260 @default.
- W2347048785 hasRelatedWork W2347048785 @default.
- W2347048785 hasRelatedWork W2353715290 @default.
- W2347048785 hasRelatedWork W2365533218 @default.
- W2347048785 hasRelatedWork W2381816536 @default.
- W2347048785 hasRelatedWork W2388981420 @default.
- W2347048785 hasRelatedWork W2389773480 @default.
- W2347048785 hasVolume "55" @default.
- W2347048785 isParatext "false" @default.
- W2347048785 isRetracted "false" @default.
- W2347048785 magId "2347048785" @default.
- W2347048785 workType "article" @default.