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- W2347084966 abstract "ABSTRACT A pH-stat method is described for determining the initial rate of efflux of H+ equivalents from trout erythrocytes after stimulation with catecholamines. This measure of transport capacity of the Na+/H+ exchanger was sensitive to medium pH with a peak at 7·24 ± 0·09 pH units. After washing blood into a trout saline the exchange capacity recovered to a maximal value after 2 h and remained stable for at least 24 h at 5 °C. Internal Na+ concentration reached a low, stable value also within 2–3 h of washing. The antiporter was comparatively unstable when incubated at room temperature. The concentrations for half-maximal responses to the agonists adrenaline, noradrenaline and isoproterenol were 3·0 ± 1·1 × 10−7, 2·5 ± 3·0 × 10−8 and 1·5 ± 0·6× 10−8moll-1, respectively. The variation of antiporter capacity with time of the year was followed over a 24month period. Capacity was highest during early summer at 200–250 mmol h-1 I-packed cell volume (pcv), but declined from November to March to a low of approximately 100mmolh-11-1 pcv. Exchange capacity increased rapidly during the early spring back to values characteristic of early summer. It appears that both temperature and photoperiod are involved in regulating the spring increase." @default.
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- W2347084966 date "1989-07-01" @default.
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- W2347084966 title "The Seasonal Modulation of Na+/H+ Exchanger Activity in Trout Erythrocytes" @default.
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- W2347084966 doi "https://doi.org/10.1242/jeb.144.1.463" @default.
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