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- W2347216830 abstract "AIM To establish the in vitro growth model of human hypertrophic scar microvascular endothelial cells(HSMEC). METHODS The human normal forskin segments and hypertrophic scar tissues obtained from clinical operations were treated with 1.25 g·L -1 dispase and then 1.25 g·L -1 trypsin solution at 4℃ for 24 hours respectively. After being released from the tissues by mechanical pressing, the human dermal microvascular endothelial cells (HDMEC) and HSMEC were cultivated in Delbeco's modified eagle medium (DMEM) supplemented with 200 mL·L -1 fetal calf serum and bFGF (5000 U·L -1 ). The cultivated MEC were isolated and purified with 0.1 g·L -1 trypsin 0.15 mmol EDTA (TE). Hematoxylin and eosin (HE) staining and immunohistochemistry staining for factor Ⅷ related antigen were performed to identify the MEC. The percentage of MEC was calculated. RESULTS MEC grew and proliferated well in DMEM containing FCS and bFGF and a few of dermal fibroblast contaminating the MEC could be removed almost completely with TE. The purities of both HSMEC and HDMEC in passage 3 were 97.0% to 98.0%, significantly higher than that in primary culture ( P 0.01). CONCLUSION A low concentration of bFGF and the TE isolation exert reliable effects on the MEC growth and purification. The successful culture of HSMEC might widen the range of study on the mechanisms of burn wound healing and scarring." @default.
- W2347216830 created "2016-06-24" @default.
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- W2347216830 date "2000-01-01" @default.
- W2347216830 modified "2023-09-27" @default.
- W2347216830 title "Isolation, purification and culture of microvascular endothelial cells from human hypertrophic scar tissue" @default.
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