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- W2347217570 abstract "GP5 gene of PRRSV contains a signal peptide and three transmembrane functional areas,and these sequences can make GP5 stay in the endoplasmic reticulum and inhibit its expression.In this report,the GP5 gene of PRRSV T1 strain separated and determined by our laboratory was optimized,GP5-ShortDNA was synthesized and the optimized gene was cloned into the expressive vector pGEX-GP5.The recombinant protein(about 41 kD) was expressed in E.coli BL21 after induction with IPTG.Western blot showed that the recombinant protein had immunological activity with the reference positive serum." @default.
- W2347217570 created "2016-06-24" @default.
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- W2347217570 date "2012-01-01" @default.
- W2347217570 modified "2023-09-26" @default.
- W2347217570 title "Optimization and Expression of GP5 Gene of PRRSV and Analysis of Immunological Activity of Its Expressed Products" @default.
- W2347217570 hasPublicationYear "2012" @default.
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