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- W2347271566 abstract "An Shanghai isolate of ribgrass mosaic virus (RMVsh) was isolated from Brassica chinensis (Qingcai).Viral RNA extracted from virus preparation was used as template for cDNA synthesis of coat protein gene by RT PCR .PCR products were purified with agarose gel and cloned into pGEM T easy vector to construct the recombinant plasmid for nucleotide sequencing.The coat protein CP gene consists of 474 nt encoding 157 amino acids. The CP gene was inserted into pBAD/His C vector and expressed in E.coli Top10 strain. The product was identified by SDS PAGE and Western blot with RMVsh antiserum.The homology of nucleotide and predicted amino acid sequences of CP gene with those of other isolates of tobamovirus infecting crucifer were 83.5%-98.9% and 87.9%-99.4%,respectively. RMVsh as a representative in first sub group of Tobamovirus infecting cruciferae plants was discussed." @default.
- W2347271566 created "2016-06-24" @default.
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- W2347271566 date "2002-01-01" @default.
- W2347271566 modified "2023-09-25" @default.
- W2347271566 title "Sequence Analysis of Coat Protein Gene of Ribgrass Mosaic Virus Shanghai Isolate and Expression in E. coli" @default.
- W2347271566 hasPublicationYear "2002" @default.
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