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- W2347295514 abstract "To optimize the preparation technology of direct vat set in industrial production, the liquid nitrogen cryogenic treatment was used in the preparation of the starter cultures of Lactobacillus paracasei L9. The viability,acid producing ability, activity of β-gal and LDH enzymes of L9 cells which were made by vacuum freezing drying technique were compared with the ones made by liquid nitrogen cryogenic treatment after determining the cryoprotectant. The results showed that the viability of L9 cells that were produced by liquid nitrogen cryogenic treatment reached 93.7±4.2%, and it was significantly higher than those produced by vacuum freezing drying technique(P0.05), which were 77.5±5.3%. Meanwhile, the acid-producing ability in skim milk of starters produced by liquid nitrogen cryogenic treatment has no significant difference with that of the untreated ones(P0.05),and it is significantly higher than those produced by vacuum freezing drying technique. The activity of β-gal and LDH enzymes of L9 cells which produced by liquid nitrogen cryogenic treatment were decreased significantly lower than those produced by vacuum freezing drying technique(P0.05).The storability of the L9 starters produced by liquid nitrogen cryogenic treatment was determined under-80℃. Results showed that the cell viability and acid-producing ability of those starters didn't significantly changed during the storage period, suggesting their good storability." @default.
- W2347295514 created "2016-06-24" @default.
- W2347295514 creator A5066037624 @default.
- W2347295514 date "2015-01-01" @default.
- W2347295514 modified "2023-09-22" @default.
- W2347295514 title "Direct Vat Set Starter of Lactobacillus paracasei L9 Made by Liquid Nitrogen Freezing" @default.
- W2347295514 hasPublicationYear "2015" @default.
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