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- W2347343992 abstract "Objective To evaluate the effects of ethyl-acetate fraction of extracts from Tetrastigma hemsleyanum Diels et.Gilg(EAFT) on the apoptotic cell death in human colon cancer HT-29 cell line.Methods Human colon cancer HT-29 cells were cultured in vitro.Flow cytometry after FITC labeled annexin V staining was used to determine the percentage of apoptotic cells.Histone/DNA fragments were determined using ELISA assay.Western Blotting was used to analyze the expresson of Bax and cyto C protein in HT-29 cells.Results EAFT significantly induced increase of the percentage of apoptotic cells(P0.05),and increased leakage of the histone/DNA fragments in HT-29 cell line(P0.05),in a concentration-dependent manner.The upregulation of the expression level of Bax and cyto c protein in HT-29 cells treated with EAFT at 10mg/L for 6,12,24 h(P0.05),as assessment by Western blotting.Conclusion EAFT can effectively induce apoptosis of human colon cancer HT-29 cells,and its mechanism is involved in activation of mitochondrial apoptotic pathway." @default.
- W2347343992 created "2016-06-24" @default.
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- W2347343992 date "2010-01-01" @default.
- W2347343992 modified "2023-09-25" @default.
- W2347343992 title "Induction of Apoptosis by Ethyl-acetate Fraction of Extracts from Tetrastigma Hemsleyanum Diels et.Gilg in Human Colon Cancer HT-29 Cells" @default.
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