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- W2347360251 abstract "To investigate the influence of codon usage optimization on the mucosal immunization with bovine papillomavirus gene L1,a live attenuated AroA, AroD autotrophic mutant of Salmonella typhimurium (S.BRD509) was used as carrier of eukaryotic expression vector (pcDNA3HBPVL1,pcDNA3WBPVL1),and inoculated into BALB/c by intraperitoneal,intranasal,intravaginal and intravenous routes. VLP-ELISA was used to detect the L1-conformational antibody and MTT method was used to observe the induced specific cell-mediated immune responses. The results showed that levels of IgG and SIgA of the mice immunized with pcDNA3HBPVL1 were higher than those of mice immunized with pcDNA3WBPVL1,but there was no any effect on cell-mediated immune responses. These results suggest that the optimized codon usage contributes to the enhancement of humoral immune responses induced by immunization with bovine papillomavirus L1 gene,and the attenuated Salmonella typhimurium may be an effective carrier for the mucosal immunization with DNA vaccine." @default.
- W2347360251 created "2016-06-24" @default.
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- W2347360251 date "2003-01-01" @default.
- W2347360251 modified "2023-09-24" @default.
- W2347360251 title "The Enhancement Effect of Codon Usage Optimization on the Mucosal Immuniza-tion with Bovine Papillomavirus Gene L1" @default.
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