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- W2347394467 abstract "【Objective】The aim of the present study was to investigate the effects of temperature and pH on digestive enzymes of Perca flavescens.【Method】Effects of temperature and pH on digestive enzymes of Perca flavescens were studied using enzymatic analysis methods.Forint phenol method was used to determine protease,while amylase and lipase were measured with amylase kit and lipase kit,respectively.【Result】The activities of all digestive enzymes in each digestive organ of Perca flavescens increased at first and then decreased with the increase of temperature and pH within the designed ranges.In stomach and liver,the optimal temperature for protease was 40℃,whereas it was 50℃in anterior intestine,middle intestine and pyloric caeca.The optimum temperature for amylase in stomach,middle intestine and liver was 35℃,while was 30℃in other organs.The optimum temperature for lipase in different digestive or-gans of Perca flavescens was 40℃.The optimum pH for protease in stomach,pyloric caeca,anterior intestine,middle intestine and liver were 2.5,8.0,9.0,9.0and 6.0,respectively.The optimum pH for amylase in stomach,pyloric caeca,anterior intestine,middle intestine and liver were 6.0,6.0,7.0,7.0and 6.0,respectively.The optimum pH for lipase in stomach was 6.0while in others were 7.0.The activities of digestive enzymes in Perca flavescens had organ specificity.Under optimum temperature,the activity of protease in different organs was in the order of pyloric caecaanterior intestinestomachmiddle intestineliver,the activity of amylase was in the order of liveranterior intestinemiddle intestinepyloric caecastomach,and the activity of lipase was in the order of liverstomachanterior intestinepyloric caecamiddle.【Conclusion】The optimum temperature for digestive enzymes of Perca flavescens was higher than its optimum growth temperature.Pepsin activity was higher in strong acidic conditions,liver protease activity was higher in acidic conditions,and intestinal and pyloric caeca protease activity was higher in alkaline conditions.Amylase activity was higher in slightly acidic conditions and the activity of lipase was higher in neutral conditions." @default.
- W2347394467 created "2016-06-24" @default.
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- W2347394467 date "2014-01-01" @default.
- W2347394467 modified "2023-09-25" @default.
- W2347394467 title "Effects of temperature and pH on activities of digestive enzymes in Perca flavescens" @default.
- W2347394467 hasPublicationYear "2014" @default.
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