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- W2347438963 abstract "In order to explore the mechanism of differences in survival ability of CD59 and CD59 bone marrow mononuclear cell (BMMNCs) among the normal controls and PNH groups the expression of Bcl-2 and Bax proteins on both normal (CD59+ ) and abnormal (CD59- ) BMMNCs in patients with PNH was observed before and after liquid culture and was compared with normal controls. The expression of Bcl-2 and Bax proteins on BMMNCs was detected by the methods of indirect immunofluorescence labeling and flow cytometry. Results showed that there were no differences in percentages of Bcl-2 and Bax positive expression cells and in values of mean fluorescence intensity (MFI) of Bcl-2 before and after liquid culture on both CD59+ and CD59- BMMNCs in PNH patients when compared with that of normal controls, while the MFI values of Bax expression on CD59+ and CD59- BMMNCs in patients were increased and became even more obvious after 7 days liquid culture, resulting in remarkable decrease of Bcl-2/Bax ratio, but no significant difference in expression of Bax was found between CD59+ and CD59- BMMNCs. It seems that the decreased survival ability of BMMNCs in PNH patients might be partly explained by the reduction of Bcl-2/Bax ratio, but the difference in viability between normal and abnormal BMMNCs might involve other mechanisms." @default.
- W2347438963 created "2016-06-24" @default.
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- W2347438963 date "1999-01-01" @default.
- W2347438963 modified "2023-09-27" @default.
- W2347438963 title "Expression of Bcl-2 and Bax on Normal and Abnormal Hematopoietic Cells in Bone Marrow of Patients with Paroxysmal Nocturnal Hemoglobinuria" @default.
- W2347438963 hasPublicationYear "1999" @default.
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