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- W2347482533 abstract "Objective:To generate mutant pools of Bacillus anthracis A16R by transposon Tn917-mediated insertional mutagenesis and screen the spo mutations.Methods:B.anthracis was transformed with temperature-sensitive plasmid pLTV3 that harbors Tn917.The culture of one transformant was diluted with fresh medium and incubated with erythromycin of low concentration in order to induce the transposition of Tn917.The mutant pools of B.anthracis was generated.The spo mutations was screened by use of triphenyl tetrazolium chloride(TTC)and fuchsin basic-methylene blue stained.And Southern hybridization was used to confirm the spo mutations.Results:Six spo mutants were screened from 2 000 Tn917 insertion mutants and they could not sporulation when growing in LB medium for five days.The probe hybridized to one EcoRⅠ fragment of each transposant,and each hybridization fragment had different molecular size.The probe did not hybridize to the wild B.anthracis DNA.Conclusions:Transposon Tn917 mutagenesis results in single chromosome insertions with apparent randomness in B.anthracis." @default.
- W2347482533 created "2016-06-24" @default.
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- W2347482533 date "2006-01-01" @default.
- W2347482533 modified "2023-09-23" @default.
- W2347482533 title "Screening the spo Mutations of Bacillus anthracis by Tn917-Mediated Insertional Mutagenesis" @default.
- W2347482533 hasPublicationYear "2006" @default.
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