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- W2347583674 abstract "Objective To investigate the α2,6-linked sialylation of colon carcinoma cell LS174T and their influence to the cell-cell aggregation of tumor cells.Methods Colon carcinoma cell LS174T were sense-transfected with ST6Gal I cDNA or antisense-transfected with a part of the ST6Gal I sequence inserted in pcDNA 3.1 vector.Tumor cells were also mock-transfected with pcDNA 3.1 vector as control.The α2,6-linked sialylation of cell surface were determined by FACS using lectin SNA(sambucus nigra agglutinin,specific for α2,6-linked sialic acid on N-linked glycoprotein).We further compare the number of cells that attached to the dish wall 4 h after inoculation.In addition,the cell-cell homotypic aggregation test of sense-,antisense-,and mock-transfectants were analyzed.Results Clones transfected with ST6Gal I in sense direction showed an enhanced ST6Gal I mRNA expression as well as an increased binding of lectin SNA to cell surface components.Sense-transfectants showed a decreased homotypic aggregation but rapid attachment to the dish wall of cell culture.On the other hand,transfection with ST6Gal I in antisense direction resulted in a reduced ST6Gal I mRNA expression,less SNA-reactivity,a significant increased homotypic aggregation but much less adherence to extracellular substances at 4h of cell inoculation.Conclusion Expression of sialyltransferase ST6Gal I of tumor cells directly affects α2,6-sialylation of cell surface and further regulates the adhesion properties of tumor cells." @default.
- W2347583674 created "2016-06-24" @default.
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- W2347583674 date "2006-01-01" @default.
- W2347583674 modified "2023-09-23" @default.
- W2347583674 title "The effect of cell surface α2,6-sialylation on the homotypic aggregation of colon carcinoma cells" @default.
- W2347583674 hasPublicationYear "2006" @default.
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