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- W2347630361 abstract "The van M sequence was amplified for V. parahaemolyticus ATCC33847 genome and inserted into p MD19-T vector for clone sequencing and sequence alignment. The correct van M fragment was double-digested by Nde I and Eco RI and ligated into the p ET22 b vector for gene expression. IPTG was used to induce van M prokaryotic expression. An acyl-HSL bio-reporter strain KYC55 was used to detect the acyl-HSL activities of ATCC33847 and Escherichia coli carrying the van M gene. HPLC-MS assay was performed to identify van M-associated acyl-HSL molecules in extracts from ATCC33847 and Escherichia coli. The ATCC33847 van M sequence was aligned with that of V. anguillarum van M, with the similarity of 57%. The expression vector p ET22b-van M was constructed and induced to produce the highest level of Van M protein by induction with 0.6mmol/L IPTG in BL21(DE3). ATCC33847 and BL21(DE3) containing p ET22b-van M presented an acyl-HSL activity, which was detected by KYC55. HPLC-MS results indicated that both 3-Hydroxybutanoyl-HSL(3-OH-C4-HSL) and 3-Hydroxydecanoyl-HSL(3-OH-C10-HSL) were detected in extracts from ATCC33847 and BL21(DE3)(p ET22b-van M). In this study, the prokaryotic expression of V. parahaemolyticus van M confirmed that V. parahaemolyticus van M was responsible for the synthesis of V. parahaemolyticus quorum sensing signals, 3-OH-C4-HSL and 3-OH-C10-HSL." @default.
- W2347630361 created "2016-06-24" @default.
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- W2347630361 date "2015-01-01" @default.
- W2347630361 modified "2023-09-26" @default.
- W2347630361 title "Research on Prokaryotic Expression of Vibrio parahaemolyticus van M Gene and N-acyl-homoserine Lactone Identification" @default.
- W2347630361 hasPublicationYear "2015" @default.
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