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- W2347649983 abstract "Background and objective The gene expressions of the two primary cell types with different morphological phenotypes,namely,cuboidal cells and polygonal cells in so-called pulmonary sclerosing hemangioma(PSH) were still unclear now.Disclosing the different gene expression of the two cell types will provide the basis for the study of histogenesis of PSH tissue and be helpful for differential diagnosis.Methods Polygonal and cuboidal cells were obtained from PSH tissue using a laser capture microdissection(LCM) technique.Total RNA was extracted and mRNA levels of cytokeratin(CK),epithelial membrane antigen(EMA),vimentin(VT),surfactant protein B(SP-B),thyroid transcription factor-1(TTF-1),synaptophysin(Syn),and chromogranin A(Ch-A) were analyzed by RT-PCR.Results The CK,EMA and SP-B genes were clearly expressed in cuboidal cells,while the VT and Syn genes were clearly expressed and the EMA gene was weakly expressed in polygonal cells.TTF-1 was expressed in both cell types,while neither cell type expressed ChA.Conclusion The differences in morphological phenotype might result from differences in the state of differentiation,cuboidal cells may differentiate into type Ⅱ pneumocytes,while polygonal cells in stroma possess the multipotency differentiation." @default.
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- W2347649983 date "2007-12-20" @default.
- W2347649983 modified "2023-09-24" @default.
- W2347649983 title "[Differential gene expressions of polygonal cells and cuboidal cells in so-called pulmonary sclerosing hemangioma]." @default.
- W2347649983 doi "https://doi.org/10.3779/j.issn.1009-3419.2007.06.04" @default.
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