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- W2347656611 abstract "Objective To construct replication-defective adenovirus containing tk gene (ADV-tk).MethodsRecombinant adenovirus of ADV-tk was constructed using homologous recombination in cells.After the interested tk gene fragment in the recombinant plasmid obtained was confirmed by PCR,the titre of purified recombinant adenovirus was detected.In vitro study,tk gene in SMMC-7721 cells transfected by ADV-tk was investigated by RT-PCR.In vivo study,ADV-tk was injected intraperitoneally into BALB/c nude mice with liver cancer and apoptosis cells in tumor were observed.Results Recombinant adenovirus containing ADV-tk was proved successfully.The titre of purified recombinant adenovirus was 1.4×1010 pfu/ml.In vitro study,tk was integrated and expressed by SMMC-7721 cells.In vivo study,with the injection of ADV-tk,apoptosis cells in tumor increased.Conclusion A replication-defective adenovirus containing tk gene is successfully constructed,which may useful for further research on tumor suicide gene therapy with ADV-tk." @default.
- W2347656611 created "2016-06-24" @default.
- W2347656611 creator A5053153606 @default.
- W2347656611 date "2010-01-01" @default.
- W2347656611 modified "2023-09-23" @default.
- W2347656611 title "Construction of Recombinant Adenovirus Containing tk Gene" @default.
- W2347656611 hasPublicationYear "2010" @default.
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