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- W2347733517 abstract "[Objective] The study aimed to develop a fast,simple Dot-Immunogold Staining(Dot-IGS) for detecting Edwardsiella tarda.[Method] Dot-IGS was established by the colloid-gold maker sheep-anti-rabbit IgG,which seleted the nitro cellulose membrane as the solid carrier.The working concentration of serum samples and colloid-gold maker sheep-anti-rabbit IgG by chessboard assay.The samples with clear and obvious dots were judged as positive reaction.[Result] Edwardsiella tarda was positive in the Dot-ELISA,while Aeromonas sobria,A.hydrophila,Vibrio fluvialis,V.alginolyticus,V.anguillarum,Shewanella putrefaciens,Providencia alcalifaciens,Enterobacter sakazakii and Escherichia coli were negative in the assay.[Conclusion] The trial indicated that the method was simple,special,quick,direct and easy extention and application at primary level." @default.
- W2347733517 created "2016-06-24" @default.
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- W2347733517 date "2009-01-01" @default.
- W2347733517 modified "2023-09-26" @default.
- W2347733517 title "Study on Rapid Detection of Pathogenic Bacteria of Ascites Disease of Paralichthys olivaceus by Dot-IGS" @default.
- W2347733517 hasPublicationYear "2009" @default.
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