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- W2347742066 abstract "Objective To investigate the specific interference of OX40 gene expression induced by RNAi technique in 293 T cell lines transfected with mice OX40 gene. Methods 293 T cells were transfected with recombined plasmid pEGFP-N1-GFP/ OX40,and then the positive cell clones were selected by fluorescence protein observation and RT-PCR. One specific dicer siRNA targeted to OX40 mRNA was designed and synthesized,sharing no homology with exons of known human gene. Quantitative real-time PCR was performed to measure the inhibitory rate of target gene expression by comparing OX40 mRNA concentrations before and after siRNA transfection. Results 10 nmol/L siRNA-OX40 elicited the highest level of gene silence in 293 T cells 48 h after siRNA transfection (76.4%); the time of maximal inhibitory effect was at 48-72 h (74.3%,38.8%). Conclusion The exogenous OX40 expression could be significantly inhibited by treatment with specific siRNA in a dose-and time-dependent manner in 293 T cells,which may provide a useful profile for further investigation on inhibition of OX40 protein,and a promising control approach for preventing immune reaction." @default.
- W2347742066 created "2016-06-24" @default.
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- W2347742066 date "2008-01-01" @default.
- W2347742066 modified "2023-09-24" @default.
- W2347742066 title "Interference of OX40 gene expression induced by small interfering RNA" @default.
- W2347742066 hasPublicationYear "2008" @default.
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