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- W2347778860 abstract "Factors such as DNA size,electric field strength and polymer character and concentration influencing the previously proposed model for quantitative DNA separation in entangled polymer solution were discussed.The selection of polymer medium instructed by the model was also evaluated.Experimental results showed that longer electrophoretic migration time was required to separate longer chain DNA,polymer with higher molecular mass was more advantageous for the separation of long chain DNA and longer migration time was required for higher concentration of polymer,moreover,higher electric field strength was advantageous for rapid separation of DNA,but should be used restrictively for efficient separation of large fragment DNA.The optimum polymer concentration for separating DNA can be computed by the model.Results also showed that high concentration polymer solution was advantageous for separating DNA.The results obtained by the experiments were consistent with those predicted by the model.The above results will be useful for promoting the study of DNA electrophoresis model and for optimizing and selecting experimental conditions for DNA separation by capillary electrophoresis with non-gel sieving matrices." @default.
- W2347778860 created "2016-06-24" @default.
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- W2347778860 date "2009-01-01" @default.
- W2347778860 modified "2023-09-23" @default.
- W2347778860 title "Optimization of the Model for Quantitative DNA Separation by Capillary Electrophoresis in Non-gel Sieving Matrices" @default.
- W2347778860 hasPublicationYear "2009" @default.
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