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- W2347781670 abstract "【Objective】To construct the eukaryotic expression vector pIRES2-EGFP-RUNX3 and to express it in human bladder cancer T24 cells. 【Methods】 The RUNX3 gene was amplified by RT-PCR from human normal bladder tissue, and was confirmed by DNA sequencing. The eukaryotic expression vector pIRES2-EGFP-RUNX3 was constructed by introducing RUNX3 DNA fragment into the pIRES2-EGFP vector, and was confirmed by EcoRI and XhoI digestion analysis. The pIRES2-EGFP- RUNX3 was transfected into human bladder cancer T24 cells using lipofectamineTM 2000. The expressed EGFP was observed under fluorescent microscope. 【Results】 The eukaryotic expression vector pIRES2-EGFP-RUNX3 was successfully constructed and transfected into human bladder cancer T24 cells. The transfected human bladder cancer displaying green fluorescence was observed under fluorescence microscope. 【Conclusions】 The eukaryotic expression vector pIRES2-EGFP-RUNX3 was constructed and EGFP could be expressed in human bladder cancer T24 cells. The present study laid the foundation for the further research of the relation of RUNX3 and human bladder cancer." @default.
- W2347781670 created "2016-06-24" @default.
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- W2347781670 date "2009-01-01" @default.
- W2347781670 modified "2023-09-22" @default.
- W2347781670 title "Construction of Eukaryotic Expression Vector pIRES2-EGFP-RUNX3 and Its Expression in Human Bladder Cancer T24 Cells" @default.
- W2347781670 hasPublicationYear "2009" @default.
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