Matches in SemOpenAlex for { <https://semopenalex.org/work/W2347796310> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W2347796310 abstract "Objective To construct and identify a conditionally replicative adenovirus(CRAd) regulated by E2F1 gene promoter.Methods Human E2F1 gene promoter and adenoviral E1A gene were amplified by PCR using the genomic DNA of human embryo kidney cells AD-293 as a template.The fragment of human E2F1 gene promoter was digested and then inserted into pEGFP-1 for cons-tructing pE2F1 p-EGFP.The open read frame sequence of E1A was digested and then cloned into pTERT promoter-EGFP to obtain pTERT p-E1A.The E1A gene was digested from recombinant pTERT p-E1A plasmid and inserted into linearized pE2F1 p-EGFP to obtain pE2F1 p-E1A.The expression cassette of E1A gene under the control of E2F1 promoter was digested from recombinant pE2F1 p-E1A plasmid and cloned into shuttle vector pDC311.The pDC311-E2F1 p-E1A plasmid was cotransfected with adenoviral genomic plasmid pBHGlox△1,3Cre into AD-293 cells to package the recombinant adenovirus Ad-E2F1 p-E1A.The Ad-E2F1 p-E1A vector was amplified and its selective replication ability was evaluated by plaque forming assay in cancer cells overexpressing E2F-1 protein. Results Positive recombinant plasmid was confirmed by restriction enzyme digestion.Ad-E2F1 p-E1A vector was successfully constructed,which could selectively replicate in E2F1 positive cancer cells. Conclusion The constructed Ad-E2F1 p-E1A in tumor cells has a good ability of selective replication,and emerge an innovative promising application in the treatment of E2F1 positive cancers." @default.
- W2347796310 created "2016-06-24" @default.
- W2347796310 creator A5040839623 @default.
- W2347796310 date "2011-01-01" @default.
- W2347796310 modified "2023-09-23" @default.
- W2347796310 title "Construction and identification of conditionally replicative adenovirus under the control of E2F1 gene promoter" @default.
- W2347796310 hasPublicationYear "2011" @default.
- W2347796310 type Work @default.
- W2347796310 sameAs 2347796310 @default.
- W2347796310 citedByCount "0" @default.
- W2347796310 crossrefType "journal-article" @default.
- W2347796310 hasAuthorship W2347796310A5040839623 @default.
- W2347796310 hasConcept C101762097 @default.
- W2347796310 hasConcept C104317684 @default.
- W2347796310 hasConcept C150194340 @default.
- W2347796310 hasConcept C153911025 @default.
- W2347796310 hasConcept C183022183 @default.
- W2347796310 hasConcept C22744801 @default.
- W2347796310 hasConcept C32470452 @default.
- W2347796310 hasConcept C33161422 @default.
- W2347796310 hasConcept C40767141 @default.
- W2347796310 hasConcept C54009773 @default.
- W2347796310 hasConcept C54355233 @default.
- W2347796310 hasConcept C83258922 @default.
- W2347796310 hasConcept C86803240 @default.
- W2347796310 hasConceptScore W2347796310C101762097 @default.
- W2347796310 hasConceptScore W2347796310C104317684 @default.
- W2347796310 hasConceptScore W2347796310C150194340 @default.
- W2347796310 hasConceptScore W2347796310C153911025 @default.
- W2347796310 hasConceptScore W2347796310C183022183 @default.
- W2347796310 hasConceptScore W2347796310C22744801 @default.
- W2347796310 hasConceptScore W2347796310C32470452 @default.
- W2347796310 hasConceptScore W2347796310C33161422 @default.
- W2347796310 hasConceptScore W2347796310C40767141 @default.
- W2347796310 hasConceptScore W2347796310C54009773 @default.
- W2347796310 hasConceptScore W2347796310C54355233 @default.
- W2347796310 hasConceptScore W2347796310C83258922 @default.
- W2347796310 hasConceptScore W2347796310C86803240 @default.
- W2347796310 hasOpenAccess W2347796310 @default.
- W2347796310 hasRelatedWork W2351825124 @default.
- W2347796310 hasRelatedWork W2352911428 @default.
- W2347796310 hasRelatedWork W2356563149 @default.
- W2347796310 hasRelatedWork W2356625414 @default.
- W2347796310 hasRelatedWork W2359841857 @default.
- W2347796310 hasRelatedWork W2362588457 @default.
- W2347796310 hasRelatedWork W2363173893 @default.
- W2347796310 hasRelatedWork W2363587206 @default.
- W2347796310 hasRelatedWork W2364346232 @default.
- W2347796310 hasRelatedWork W2364445291 @default.
- W2347796310 hasRelatedWork W2368157655 @default.
- W2347796310 hasRelatedWork W2372658473 @default.
- W2347796310 hasRelatedWork W2374745218 @default.
- W2347796310 hasRelatedWork W2376271110 @default.
- W2347796310 hasRelatedWork W2377849370 @default.
- W2347796310 hasRelatedWork W2381061703 @default.
- W2347796310 hasRelatedWork W2386309805 @default.
- W2347796310 hasRelatedWork W2391463707 @default.
- W2347796310 hasRelatedWork W2391960765 @default.
- W2347796310 hasRelatedWork W3031141790 @default.
- W2347796310 isParatext "false" @default.
- W2347796310 isRetracted "false" @default.
- W2347796310 magId "2347796310" @default.
- W2347796310 workType "article" @default.