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- W2347800008 abstract "To explore the influence of water temperature and 17β-estradiol( E2) on the growth of the Chinese giant salamander larvae,Andrias davidianus,and to further reveal the mechanism of E2effect on the growth of the larvae,eighteen groups formed by factors interaction among three water temperatures,two E2concentrations and three exposure periods,were analyzed. There were totally twenty one groups in the experiment when considering three control groups without E2at different water temperatures. Three water temperatures were 1-16℃( drawing natural river water into experimental cement pool),( 13 ± 1) ℃ and( 20 ± 1) ℃; two E2concentrations were 25 μg /L and 250 μg /L; and three E2exposure periods were 40,80 and 120 days,respectively. The post-hatching 60-day larvae were selected for experiment,and twenty larvae were fed in the cement pool which contained 40 liters of water as a group. The weight of the larvae was calculated at 143,182and 248 days after post-hatching. One-way ANOVA and LSD multiple comparison analysis were used to average weight of the larvae in different groups. In addition,eight post-hatching 143-day larvae exposed to E2for 40days were randomly chosen from control,25 μg /L E2and 250 μg /L E2groups at( 20 ± 1) ℃,and the livers for microstructure observation. The results showed that the growth of larvae was fast at( 20 ± 1) ℃,less fast at( 13± 1) ℃,and the slowest at range of 1- 16℃( P 0. 01,Fig. 1 and 2). E2had adverse effects on the growth of larvae at( 20 ± 1) ℃; in addition,the toxicity of E2increased with the exposure time and concentration( P 0. 01,Fig. 1,2 and 3); vacuolization in hepatic cells and enlargement in hepatic sinusoid were observed in the liver at 25 μg /L E2( Fig. 4b). Moreover,epithelium cells in the liver capsule migrated into the inner tissue in 250 μg /L E2group( Fig. 4c). The growth of larvae might be inhibited by liver damage,which was probably caused by E2." @default.
- W2347800008 created "2016-06-24" @default.
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- W2347800008 date "2014-01-01" @default.
- W2347800008 modified "2023-09-28" @default.
- W2347800008 title "Water Temperature and Estradiol Affect the Growth and Liver of the Chinese Giant Salamander Larvae,Andrias davidianus" @default.
- W2347800008 hasPublicationYear "2014" @default.
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