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- W2347818078 abstract "Objective To obtain short peptides mimicking antigenic epitopes of the membrane antigen from Schistosoma japonicum hepato-portal schistosomula (SjHmAg), and to explore their protective immunity against Schistosoma japonicum (Sj) in mice. Methods IgG antibodies were purified from rabbit sera immunized with SjHmAg. The purified IgG was used to immunoscreen a phage random peptide library of 12 amino acids. Three rounds of biopanning were carried out, and 24 randomly selected positive clones were detected by ELISA and sequenced. Kunming mice were repeatedly immunized with mixed phage clones. Each mouse was challenged 40±1 Sj cercariae, and all mice were perfused 42 days after challenge. The worms and the eggs per gram liver tissue were counted. Results The specific phages binding to IgG were enriched 210 folds after three rounds of biopanning. Twenty-four clones were detected by ELISA, 21 of them were shown to actually bind to the specific IgG and sequenced, 7 clones had the core sequence (STRKD). Compared with the control groups. The reduction rates of the worm burden and liver egg were 16.15% and 59%, respectively. Conclusion The specific peptides mimicking epitopes of SjHmAg can be obtained by immunoscreening phage random peptide library and a protective immunity against Sj can be stimulated by these epitopes in mice." @default.
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- W2347818078 date "2003-06-21" @default.
- W2347818078 modified "2023-09-25" @default.
- W2347818078 title "A short peptide mimicking epitopes of the membrane antigen of Schistosoma japonicum hepato-portal schistosomula using phage display techniques" @default.
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