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- W2347822923 abstract "Objective To establish bladder cancer cell line with stable high expression of Uroplakin II (UPII) gene.Methods The eukaryotic expression vector containing full-length UPII gene was constructed by molecular cloning techniques,analyzed by restrictive digestion reaction and nucleic acid sequencing.Under the induction of cation liposome Lipofectamine 2000,the vector was transferred into bladder transitional cell carcinoma (TCC) cell line EJ,which lacking UPII gene expression.After being selected by G 418,the subclone cell line was obtained.Cellular gene expression of UPII was identified by RT-PCR and Western blotting.Results After EJ cells being transfected by constructed eukaryotic expression vector pcDNA3-UPII,the subclone cell line EJ/UPII was obtained by persistent G 418 selection for 4 weeks.RT-PCR and Western blotting revealed that there was no UPII expression in EJ cells,while high mRNA and protein expression of UPII was significantly detected in EJ/UPII cells (P 0.01).Conclusion The bladder TCC cell line with stable high expression of UPII gene has been successfully established through gene transfection method,which was a basis for further exploring the roles of UPII gene in TCC biological behaviors and potential strategies for targeted biological therapy of TCC." @default.
- W2347822923 created "2016-06-24" @default.
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- W2347822923 date "2004-01-01" @default.
- W2347822923 modified "2023-09-23" @default.
- W2347822923 title "Establishment of human bladder cancer cell line stably expressing Uroplakin II gene and its significance" @default.
- W2347822923 hasPublicationYear "2004" @default.
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