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- W2347823341 abstract "In order to make the simultaneous detection of PRRV and C.perfringens,a duplex RT-PCR method was established.The RNA of PRRV and DNA of C.perfringens from animal tissues were extracted by modified extraction method.Two pairs of specific primers were designed according to the N gene of PRRV andαtoxin gene of C.perfringens.Based on optimizing PCR reaction condition,the duplex RTPCR assay was developed,and two bands of 406bp(PRRV)and 272bp(C.perfringens)emerged on the gel simultaneously.The relative sensitivity and specificity of the duplex RT-PCR were evaluated.The results showed that the method was specific,sensitive and stable,and no specific band could be amplified from other pathogens.The sensitivity of the assay was 0.001TCID50/mL and 1CFU/mL,respectively." @default.
- W2347823341 created "2016-06-24" @default.
- W2347823341 creator A5055680475 @default.
- W2347823341 date "2015-01-01" @default.
- W2347823341 modified "2023-09-25" @default.
- W2347823341 title "Establishment of a Duplex RT-PCR for Detecting Peste Des Petits Ruminants Virus and C.perfringensin Sheep and Goats" @default.
- W2347823341 hasPublicationYear "2015" @default.
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