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- W2347865774 abstract "The concentration of progesterone receptors in the cytosol and nucleus of rat uterus was measured during the implantation phase of pregnancy in relation to plasma progesterone concentration. Pure strain female Wistar rats with normal oestrous cycles were used. Uterine tissues were homogenized and separated by centrifugation, Cytoplasmic and nuclear fractions were incubated with 3H-progesterone and excess cortisol. Eeeep-tor bound 3H-progesterone was separated from free 3H-progesterone by column ehroma-tography on Sephadex LH-20 in cytosolic assay. Absolute alcohol was used to extract 3H-progesterone in nuclear assay. Plasma progesterone was assayed by RIA. DNA was measured by Burton's technique and protein in the cytoplasm by the method of Lowry et al. Plasma progesterone concentration of day 1 (D1) rat was 44 n mol/1, increased on D3 to 113 n mol./l (p0.001) with synchronous increase of nuclear receptor on D2 (4.26 p mol/1), as compared with D1 (2.48 pmol./mg DNA) with P0.001 and simultaneous decrease of cytosol progesterone receptors from 122 f mol/mg protein on D2 to 75 f mol/mg protein on D3, indicating uterine preparation for implantation on D5. Concentration of plasma progesterone, cytosol and nuclear progesterone receptors all increased on D6, signifying changes of post-implantation." @default.
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- W2347865774 date "1985-01-01" @default.
- W2347865774 modified "2023-09-25" @default.
- W2347865774 title "PROGESTERONE RECEPTORS IN THE RAT UTERUS DURING THE IMPLANTATION PHASE OF PREGNANCY (DAYS 1-6)" @default.
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