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- W2347914788 abstract "Three-frame cDNA expression library was constructed using mRNA of B Lymphoid cells from the bursa of Fabricius of 3 week-old SPF chicken. cDNA were synthesized using biotin-conjugated Oligo (dT) primer in the 5'end. The double- strand cDNA was ligated to three-frame adapter and passed the cDNA Size Fractionation Columns. The cDNA entry libraries were constructed by BP recombination. The libraries have a high titer of 1×105 CFU/mL, 1×105 CFU/mL, 1.2×105 CFU/mL, respe- ctively, and contains a total clones of 1.2×106 CFU, 1.2×106 CFU, 1.5×106 CFU with an average inserts size of about 1 270 bp, 1 190 bp and 1 120 bp respectively. The constructed cDNA expression library by LR recombination has a titer of 1×105 CFU/mL and contains total clones of 1.2×106 CFU, with an average inserts size of 1 017 bp. The three-frame cDNA expression libraries would be a valuable resource for identification of cell receptors of IBDV on the chicken B cells and its cellular tropism." @default.
- W2347914788 created "2016-06-24" @default.
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- W2347914788 date "2008-01-01" @default.
- W2347914788 modified "2023-09-27" @default.
- W2347914788 title "Construction of three-frame cDNA expression library of B Lymphoid cells in the bursa of Fabricius" @default.
- W2347914788 hasPublicationYear "2008" @default.
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