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- W2347948898 abstract "To detect the immune effect of eukaryotic expression plasmid cloned with goose parvovirus(GPV) VP3 gene in mice.Eukaryotic expression vector with goose parvovirus(GPV) VP3 gene(pVAXⅠ/VP3) constructed in our laboratory and empty vector plasmid pVAXⅠwere extracted abundantly,two groups of BALB/c mice were injected by i.m.with these two plasmids at the two points respectively and immunized for four times.The T lymphocyte proliferation activity was detected by MTT,serum GPV specific antibody of the mice were detected by indirect ELISA.T lymphocyte proliferation test showed no significant difference between the indexes of immunized mice and the normal mice.The result of indirect ELISA showed that the serum GPV specific antibody level of mice immunized with pVAXⅠ/VP3 plasmid improved significantly comparing with those of mice in empty vector control group and negative control group.That indicated that the constructed eukaryotic expression vector with GPV VP3 gene induced significant humoral immunity.The research made a foundation for developing goose plaque DNA vaccine." @default.
- W2347948898 created "2016-06-24" @default.
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- W2347948898 date "2008-01-01" @default.
- W2347948898 modified "2023-09-25" @default.
- W2347948898 title "Immune Effect of Eukaryotic Expression Plasmid with Goose Parvovirus(GPV) VP3 Gene in Mice" @default.
- W2347948898 hasPublicationYear "2008" @default.
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