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- W2347949272 abstract "Objective To detect prtC gene and its expression product (PrtC) of P.gingivalis in subgingival plaques from chronic periodontitis patients and to elucidate the correlation between PrtC levels and periodontal lesions, and to identify the effect of PrtC on inducing host cell to secrete inflammatory cytokines. Methods SDS-PAGE was used to monitor the recombinant PrtC (rPrtC) expression of prokaryotic expression system and the purity of rPrtC extracted by Ni-NTA affinity chromatography. Rabbits were immunized with rPrtC to obtain antiserum. A multiple PCR was established to detect 16S rDNA and prtC genes of P.gingivalis in the subgingival plaque samples from 209 teeth sites of 56 chronic periodontitis patients. ELISA method was also established to examine PrtC in the samples and the correlation between the PrtC levels and periodontal lesions. The effects of rPrtC on inducing human umbilical vein endothelial cell line EVC-304 to secrete IL-1α, IL-8 and TNF-α. Results rPrtC output was approximate 50% of the total bacterial proteins. The extracted rPrtC after SDS-PAGE only showed a single fragment, 96.1% (201/209) and 92.3% (193/209) of the subgingival plaque samples were P.gingivalis 16S rDNA and prtC genes positive confirmed by PCR. 91.4% of the subgingival plaque samples (191/209) were PrtC ELISA positive. The PrtC levels in the subgingival plaque samples from advanced periodontitis were significantly higher than those in the samples from mild and moderate peridontitis (P0.05). No difference of the PrtC level was found between the samples from mild/moderate peridontitis and the samples from moderate/advanced peridontitis (P0.05). After co-incubation with 1 μg rPrtC for 24 h and with 5 or 10 μg rPrtC for 12 h, [CM(43]the levels of IL-1α, IL-8 and TNF-α secreted by the EVC-304 cells significantly increased (P0.05). Conclusion[CM)] P.gingivalis has high carrying and expression frequencies of prtC gene. The lesions of chronic periodontitis are closely relative to the subgingival PrtC levels. rPrtC has the activity to directly induce host cells to synthesize and secrete IL-1α, IL-8 and TNF-α." @default.
- W2347949272 created "2016-06-24" @default.
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- W2347949272 date "2006-01-01" @default.
- W2347949272 modified "2023-09-26" @default.
- W2347949272 title "Correlation between the level of Porphyromonas gingivalis collagenase in subgingival plaques of chronic periodontitis and the periodontal lesions" @default.
- W2347949272 hasPublicationYear "2006" @default.
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