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- W2347979737 abstract "OBJECTIVE:To screen anoikis resistance-associated genes by gene array and investigate the expression and clinical significance of TC-1 gene in suspension culture A549 cell line.METHODS:The differential expressions of genes in suspension culture and attachment culture A549 cell line were compared by gene array,and NCBI Pubmed was used to find the genes which had correlations with anoikis resistance in lung cancer and were differentially expressed in the chip,to screen the most changed gene among these genes and determine the gene was expressed or not by reverse transcription-polymerase chain reaction(RT-PCR)and Western blotting.RESULTS:The differential expressions were detected in 745 genes by gene array,and 63 genes were correlated with anoikis resistance in lung cancer,among which the most differential expression changed gene-TC-1 was gained.The expressions of TC-1 gene and its encoded protein in suspension culture A549 cell line were significant higher than in attachment culture A549 cell line(P0.02).CONCLUSIONS:Gene array technique provides a promising approach to the specific gene expression of the presence of anoikis resistance in lung cancer.TC-1 gene might play a role in the anoikis resistance process of lung cancer,and it might also correlate with the invasion and metastatic behavior of lung cancer cells." @default.
- W2347979737 created "2016-06-24" @default.
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- W2347979737 date "2010-01-01" @default.
- W2347979737 modified "2023-09-23" @default.
- W2347979737 title "Investigation on gene expression pattern of anoikis resistance lung cancer A549 cell line and expression and significance of TC-1 gene" @default.
- W2347979737 hasPublicationYear "2010" @default.
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