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- W2348045209 abstract "Based on the cDNA sequence of BcMF4 related to male sterility of Chinese cabbage-pak-choi(Brassica campestris ssp.chinensis var.communis,syn.B.rapa ssp.chinensis var.communis),a pair of specific primers were designed and a 607 bp fragment was amplified from the cDNA of flower buds of Chinese cabbage-pak-choi.The fragment was introduced to binary vector pBI121,and the generated antisene RNA vector was then mobilized into Agrobacterium tumefaciens strain LBA4404.The A.tumefaciens harboring the BcMF4 fragment was transformed to flowering Chinese cabbage(B.campestris ssp.chinensis var.parachinensis) via tissue culture,and 12 independent transgenic plants were obtained.Approximately 43.7% of the pollen grains from 5 transgenic plants exhibited abnormal shapes,and only 24.7% of pollen grains from these plants germinated normally.These results indicated that the functional interrupted by antisense RNA resulted in partial pollen grains abortion in flowering Chinese cabbage,suggesting that the product of BcMF4 gene plays an important role during pollen development of Chinese cabbage crops such as Chinese cabbage-pak-choi and flowering Chinese cabbage." @default.
- W2348045209 created "2016-06-24" @default.
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- W2348045209 date "2007-01-01" @default.
- W2348045209 modified "2023-09-23" @default.
- W2348045209 title "Functional validation of BcMF4 gene related to male sterility of Chinese cabbage by antisense RNA" @default.
- W2348045209 hasPublicationYear "2007" @default.
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