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- W2348055853 abstract "Objective To compare PEG precipitation method and mashing method without PEG in viral PCR detection in seafoods. Methods Commercial hepatitis A vaccine as standard substance were diluted to various concentrations with gradient method. Then seafoods were contaminated with the hepatitis A vaccine,and the viral nucleic acid was extracted following treatment by pretreatment method for hepatitis A vaccine detection of GB / T22287- 2008 and PBS mashing digestive glands,then fluorescent PCR was employed for qualitative analysis and semi- quantitative detection of hepatitis A virus severally,finally the difference of the two different pretreatment methods were compared. Results Hepatitis A virus could be detected by both methods. The lgCCID 50 of the highest dilution ratio were 1,2 and 4 for vaccine solution,sample pretreated by PEG precipitation method and that by mashing method without PEG. Conclusion Pretreatment by PEG precipitationt method is better for virus PCR detection in seafoods due to easy operation and better sensitivity." @default.
- W2348055853 created "2016-06-24" @default.
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- W2348055853 date "2014-01-01" @default.
- W2348055853 modified "2023-09-27" @default.
- W2348055853 title "Pretreatment methods comparison of virus PCR detection in seafoods" @default.
- W2348055853 hasPublicationYear "2014" @default.
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