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- W2348064727 abstract "Aim To clone the catalytic domain gene of human caspase-8 and two kinds of human caspase-8 genes which their large subunits were preceded by their small subunits were reconsctructed . Expression of the reconstructed human caspases-8 genes in HeLa cells and their effects on growth of HeLa cells were evaluated.Methods The catalytic domain gene of human caspase-8 was cloned by RT-PCR, two kinds of reconstructed human caspase-8 genes which their large subunits were proceded by their small subunits were reconstructed by recombinant PCR. The reconstructed human caspases-8 genes were cloned into eukaryotic expression vector pIRES2-EGFP. HeLa cells transfected with reconstructed human caspases-8 were observed under fluorescent and reverse electronic microscopes. Results The catalytic domain gene of human caspase-8 was cloned by RT-PCR, three kinds of reconstructed human caspases-8 genes and their eukaryotic expression vectors were reconstructed successfully. After being transfected, HeLa cell appeared cell death when the reconstructed human caspase-8 genes expressed.ConclusionThe expression of reconstructed human caspases-8 genes can accelerate HeLa cell death efficiently." @default.
- W2348064727 created "2016-06-24" @default.
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- W2348064727 date "2002-01-01" @default.
- W2348064727 modified "2023-09-23" @default.
- W2348064727 title "Expression of recombinant human caspase-8 genes and their effects on growth of HeLa cells" @default.
- W2348064727 hasPublicationYear "2002" @default.
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