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- W2348081878 abstract "Objective:To develop a strain of vaccine in Attenuated Salmonella typhimurium SL3261 harboring recombinant expressing vector of rat 4-1BBL gene.Methods:pIRES2-EGFP-4-1BBL vector was converted to LB5000(Salmonella typhimurium)for methylation and then the modified eukaryotic vector was electrotransfered to final host SL3261,screened single colonies by antibiotics was tested through plasmid extraction and PCR.Meanwhile the reconstructed SL3261 or its predecessor was cocultured with HepG2 cells to detect their invasion.Fluorescence microscope observasion and RT-PCR was exploited to observe GFP and 4-1BBL mRNA in the host cells.Results:The similar size of originated plasmids was extracted from strains SL3261 imported by the pIRES2-EGFP-4-1BBL plasmids,which was amplified as a 930 bp fragment by PCR.The capacity of SL3261 to invade HepG2 cells was 201±46 CFU/200 HepG2 cells,whereas SL3261-pIRES2-EGFP was 163±37 CFU/200 HepG2 cells and SL3261-pIRES2-EGFP-4-1BBL was 158±32 CFU/200 HepG2 cells(P0.05).The transfected HepG2 cells by infection of SL3261 containing the vector were shown with GFP and the 930 bp target expression by RT-PCR.Conclusion:Attenuated Salmonella typhimurium SL3261 containing recombined eukaryotic expressing plasmid pIRES2-EGFP-4-1BBL is successfully constructed which can deliver recombinant plasmid into HepG2 cells." @default.
- W2348081878 created "2016-06-24" @default.
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- W2348081878 date "2009-01-01" @default.
- W2348081878 modified "2023-10-07" @default.
- W2348081878 title "Construction and indentification of the recombinant vaccine of Attenuated Salmonella typhimurium containing pIRES2-EGFP-4-1BBL vector" @default.
- W2348081878 hasPublicationYear "2009" @default.
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