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- W2348098678 abstract "A competitive enzyme immunoassay with monoclonal antibody was established for measuring β-adrenergic agonist clenbuterol hydrochloride in urine and feed.The CL-Kit showed good sensitivity with the IC_(50) of 7.94 μg/L toward CL,(1 000) μg/L toward salbuterol and more than (6 400) μg/L towards norepinephrine,epinephine,isoprenaline and ractopamine.CL-Kit was performed with standard CL competitor,which produced sigmoidal like curves fitted to the four parameter logistic equation with the linear detection of 0.5-128 μg/L(R~2=0.996) and the variation coefficient of 2.3%-3.6%.The CL-Kit generally had 0.79% cross-reactivity towards salbuterol,and showed little or no cross-reactivity towards norepinephrine,epinephine,isoprenaline,ractopamine,antibiotics and multi-vitamines added in feeds.The CL-Kit also showed fine precision and accuracy of assay in shelf life.As expected,the recoveries of CL spiked in pig urine were 91%-105%.The inter-assay coefficient variation was below 20%.The intra-assay CV was below 10%." @default.
- W2348098678 created "2016-06-24" @default.
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- W2348098678 date "2004-01-01" @default.
- W2348098678 modified "2023-09-25" @default.
- W2348098678 title "Development of Monoclonal Antibody Enzyme Immunoassay for β-Adrenergic Agonist Clenbuterol Hydrochloride" @default.
- W2348098678 hasPublicationYear "2004" @default.
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