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- W2348110842 abstract "Objective:To develop a pattern for transgenic animal mediate by lentiviral vector.Methods:The research amplified the attB1-EGFP-attB2 sequence by PCR,and constructed pLenti6/v5-DEST-EGFP vector by Gateway technology.Lentiviral delivered for packing cell line by four-vector system transfection with the mediation of calcium phosphate.Purifying and collecting lentiviral and infecting cells line for determination of titer.Results:By sequencing and analyzing the lentiviral vector sequence,we certificated the vector can effective expression,and detected the titer of lentiviral is 2×107TU/mL.The EGFP was expressed with a better effect when lentivirus infecting chicken blastodermal cells.Conclusion:The lentiviral is infectable to animal cell,and the present work was useful for lentiviral vector to be used as a tool in transgenic birds." @default.
- W2348110842 created "2016-06-24" @default.
- W2348110842 creator A5089174140 @default.
- W2348110842 date "2007-01-01" @default.
- W2348110842 modified "2023-09-24" @default.
- W2348110842 title "Construction of Lentiviral Vectors by Gateway Method and Its Expression in Chicken Blastodermal Cell" @default.
- W2348110842 hasPublicationYear "2007" @default.
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