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- W2348146827 abstract "This paper tells how the wheatgrass tissue culture and regeneration system with the immature inflorescence as explant was established using four species, namely: A. mongolicum Keng, A.cristatum cv. Fairway, A.desertorum cv. Nordan, and A.cristatum×A.desertorum cv. Hycrest-Mengnong as materials. Based on the established regeneration system, the P5CS gene, which regulates the last step of key enzymes mutant gene of proline synthesis in a plant, was transformed into wheatgrass with phosphinothricin acetyltransferase (bar) conferring herbicide resistance as selecting gene. The transformation was conducted through microprojectile bombardment of callus derived from immature inflorescence.The CBF4 gene and its promoter had been isolated from Arabidopsis thaliana by PCR. The sequence analysis and function assay were conducted.The primary results and progress are summarized as follows:1. The inflorescence of a length of 1.0 to 3.0 cm is optimal to be used as explant to induce the callus. The feasible medium for callus induction from immature inflorescence was the improved MS+2,4-D (2.0 mg/L). The induction frequency was above 83.5%. The differentiation medium was MS+KT (0.2 mg/L), with a differentiation ratio of 74.5%. The rooting medium was 1/2 MS with 100% rooting ratio.2. The transgenic plants were obtained by microprojectile bombardment of callus induced from immature inflorescence,the results of PCR and Southern analysis displayed that the exogenous P5CS gene had integrated into the genome of transgenic wheatgrass,and the assay of RT-PCR showed that the transgenic P5CS had expressed at a transcript level. The transgenic frequencies of P5CS gene were 0.09%～0.11% in 2003.3. The CBF4 gene and its promoter were isolated from Arabidopsis thaliana by PCR. The analysis of CBF4 promoter sequence with bioinformatics software indicated that it could be one of the inducible promoters by outside environmental stresses. The GUS expression cassette driven by CBF4 promoter was constructed and transformed into tabacco(Wisconsin 38). The assay of the expression of GUS in the transgenic tobacco displayed that CBF4 promoter could be induced by drought stress, in accordance with the analysis with bioinformatic software." @default.
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- W2348146827 date "2005-01-01" @default.
- W2348146827 modified "2023-09-25" @default.
- W2348146827 title "Study on Culture and Regeneration of Wheatgrass and Its Genetic Transformation for Drought Tolerance" @default.
- W2348146827 hasPublicationYear "2005" @default.
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