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- W2348209138 abstract "According to the sequences of the 793/B serotype of IBV S1 gene published in GenBank,a pair of primers was designed and synthesized to specifically amplify the 891 bp nucleotide fragment,the RT-PCR technique for detection of the 793/B serotype of IBV was established.The 891 bp special strip was found only in the PCR amplification of the 793/B serotype of IBV in the specificity assay,there were no the same strip appeared in the other viruses and strains of IBV.The sensitivity result indicated that as little as 10 pg in sample were detected in PCR.Seven of the eight uncertain isolates detected by the established RT-PCR technique were positive.The above results showed that the technique provided a more sensitive,specific and rapid method for diagnosis and epidemiological survey of the 793/B serotype of IBV." @default.
- W2348209138 created "2016-06-24" @default.
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- W2348209138 date "2007-01-01" @default.
- W2348209138 modified "2023-09-27" @default.
- W2348209138 title "Establishment and application of RT-PCR technigue for detection of the 793/B serotype of infectious bronchitis virus" @default.
- W2348209138 hasPublicationYear "2007" @default.
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